上皮性钠离子通道（ENaC）的α、β和γ亚基在功能上与大鼠肝细胞中高渗诱导的阳离子通道（HICC）相关。
Subunits alpha, beta and gamma of the epithelial Na+ channel (ENaC) are functionally related to the hypertonicity-induced cation channel (HICC) in rat hepatocytes.
作者信息
Plettenberg Sandra, Weiss Eike C, Lemor Robert, Wehner Frank
机构信息
Max-Planck-Institut für molekulare Physiologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany.
出版信息
Pflugers Arch. 2008 Mar;455(6):1089-95. doi: 10.1007/s00424-007-0355-7. Epub 2007 Oct 10.
Specific small interfering RNA (siRNA) constructs were used to test for the functional relation of subunits alpha, beta, and gamma of the epithelial Na(+) channel (ENaC) to the hypertonicity-induced cation channel (HICC) in confluent rat hepatocytes. In current-clamp recordings, hypertonic stress (300 --> 400 mosM) increased membrane conductance from 75.4 +/- 9.4 to 91.1 +/- 11.2 pS (p < 0.001). The effect was completely blocked by 100 microM amiloride and reduced to 46, 30, and 45% of the control value by anti-alpha-, anti-beta-, and anti-gamma-rENaC siRNA, respectively. Scanning acoustic microscopy revealed an initial shrinkage of cells from 6.98 +/- 0.45 to 6.03 +/- 0.43 pl within 2 min. This passive response was then followed by a regulatory volume increase (RVI) by 0.42 +/- 0.05 pl (p < 0.001). With anti-alpha-, anti-beta-, and anti-gamma-rENaC siRNA, the volume response was reduced to 31, 31, and 36% of the reference level, respectively. It is concluded that all three subunits of the ENaC are functionally related to RVI and HICC activation in rat hepatocytes.
使用特异性小干扰RNA（siRNA）构建体来测试上皮钠通道（ENaC）的α、β和γ亚基与汇合大鼠肝细胞中高渗诱导阳离子通道（HICC）的功能关系。在电流钳记录中，高渗应激（300 --> 400 mosM）使膜电导从75.4±9.4 pS增加到91.1±11.2 pS（p < 0.001）。该效应被100 μM氨氯吡咪完全阻断，并且分别被抗α-rENaC、抗β-rENaC和抗γ-rENaC siRNA降低至对照值的46%、30%和45%。扫描声学显微镜显示细胞在2分钟内从6.98±0.45 pl初始收缩至6.03±0.43 pl。这种被动反应随后是0.42±0.05 pl的调节性容积增加（RVI）（p < 0.001）。使用抗α-rENaC、抗β-rENaC和抗γ-rENaC siRNA时，容积反应分别降低至参考水平的31%、31%和36%。结论是，ENaC的所有三个亚基在功能上与大鼠肝细胞中的RVI和HICC激活相关。
Zotero 插件