Wu Yu-jia, Jiang Hua, Zhou Guang-dong, Xia Wan-yao, Luo Xu-song, Liu Xia
Department of Plastic Surgery, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China.
Zhonghua Zheng Xing Wai Ke Za Zhi. 2007 Jul;23(4):328-31.
To test the hypothesis that tissue-engineered cartilage can be bioincorporated with a nonreactive, permanent endoskeletal scaffold.
Chondrocytes obtained from swine articular were seeded onto polyglycolic acids(PGA) scaffold which was incorporated with high-density polyethylene (Medpor). After cultured in vitro for two weeks,the cell-scaffold construct was implanted into subcutaneous pockets on the back of nude mice. Six weeks later,the newly formed cartilage prosthesis was harvested, and a small part of sample was evaluated by gross view, histology, type II collagen immunohistochemistry and biochemistry. PGA scaffold seeded with cells as the control group.
The newly formed cartilage was very similar to normal cartilage in both gross view and histology, and jointed Medpor tightly. The center of control group was hollow.
This pilot technique combining tissue engineering with a permanent success in creating cartilage without "hollow" phenomenon. biocompatible endoskeleton demonstrated
验证组织工程软骨能否与无反应性的永久性内骨骼支架生物融合的假说。
将从猪关节获取的软骨细胞接种到与高密度聚乙烯（Medpor）结合的聚乙醇酸（PGA）支架上。体外培养两周后，将细胞-支架构建体植入裸鼠背部的皮下囊袋中。六周后，收获新形成的软骨假体，一小部分样本通过大体观察、组织学、II型胶原免疫组化和生物化学进行评估。接种细胞的PGA支架作为对照组。
新形成的软骨在大体观察和组织学上均与正常软骨非常相似，并与Medpor紧密连接。对照组中心为中空。
这项将组织工程与永久性生物相容性内骨骼相结合的初步技术成功创建了无“中空”现象的软骨。
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