经典猪瘟病毒E(rns)糖蛋白在对抗新城疫病毒（NDV）介导的IFN-β诱导中的新作用。

A novel role of classical swine fever virus E(rns) glycoprotein in counteracting the newcastle disease virus (NDV)-mediated IFN-beta Induction.

作者信息

Xia Yan-hua, Chen Liu, Pan Zi-shu, Zhang Chu-yu

机构信息

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, China.

出版信息

J Biochem Mol Biol. 2007 Sep 30;40(5):611-6. doi: 10.5483/bmbrep.2007.40.5.611.

DOI:10.5483/bmbrep.2007.40.5.611

PMID:17927891

Abstract

E(rns) is an envelope glycoprotein of classical swine fever virus (CSFV) and has an unusual feature of RNase activity. In the present study, we demonstrate that E(rns) counteracts Newcastle disease virus (NDV)-mediated induction of IFN-beta. For this purpose, E(rns) fused to the enhanced green fluorescent protein (EGFP) was transiently expressed in porcine kidney 15 (PK15) cells. In luciferase activity assay, E(rns)-EGFP was found to prevent IFN-beta promoter-driven luciferase expression and block the induction of IFN-beta promoter mediated by NDV in a dosedependent manner. Through IFN-specific semi-quantitative RT-PCR detection, obvious decrease of IFN-beta mRNA in NDV-infected PK15 cells was observed in the presence of E(rns)-EGFP. In contrast, EGFP alone showed none of this block capacity. In addition, E(rns)-EGFP mutations with RNase inactivation were also found to block NDV-mediated induction of IFN-beta. These evidences establish a novel function for CSFV E(rns) glycoprotein in counteraction of the IFN-beta induction pathway.

摘要

E(rns)是经典猪瘟病毒（CSFV）的一种包膜糖蛋白，具有核糖核酸酶活性这一独特特征。在本研究中，我们证明E(rns)可对抗新城疫病毒（NDV）介导的β干扰素诱导。为此，与增强型绿色荧光蛋白（EGFP）融合的E(rns)在猪肾15（PK15）细胞中瞬时表达。在荧光素酶活性测定中，发现E(rns)-EGFP可阻止β干扰素启动子驱动的荧光素酶表达，并以剂量依赖方式阻断NDV介导的β干扰素启动子诱导。通过干扰素特异性半定量逆转录-聚合酶链反应检测，在存在E(rns)-EGFP的情况下，观察到NDV感染的PK15细胞中β干扰素信使核糖核酸明显减少。相比之下，单独的EGFP没有这种阻断能力。此外，核糖核酸酶失活的E(rns)-EGFP突变体也被发现可阻断NDV介导的β干扰素诱导。这些证据确立了CSFV E(rns)糖蛋白在对抗β干扰素诱导途径中的新功能。