Yoshimatsu Miho, Toyoda Atsushi, Onizawa Naoki, Nakamura Yutaka, Minato Hajime
United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Japan.
Biosci Biotechnol Biochem. 2007 Oct;71(10):2577-80. doi: 10.1271/bbb.70290. Epub 2007 Oct 7.
The cellulose-binding proteins, CBPA and CBPB, of rumen cellulolytic bacterium Eubacterium cellulosolvens 5 were biochemically characterized, and their properties were compared. Recombinant CBPA and CBPB were a typical 1,4-beta-endoglucanase. Both proteins bound to insoluble polysaccharides such as Avicel cellulose, acid swollen cellulose, lichenan, chitin, and oat spelt xylan. On the other hand, only recombinant CBPB bound to agarose and starch.
对瘤胃纤维素分解菌溶纤维真杆菌5的纤维素结合蛋白CBPA和CBPB进行了生化特性分析，并比较了它们的特性。重组CBPA和CBPB是典型的1,4-β-内切葡聚糖酶。这两种蛋白都能与不溶性多糖结合，如微晶纤维素、酸溶纤维素、地衣多糖、几丁质和燕麦麸木聚糖。另一方面，只有重组CBPB能与琼脂糖和淀粉结合。
Zotero 插件