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添加麦芽糊精-β-环糊精对香蕉汁酶促褐变活化作用的动力学研究

Kinetic study of the activation of banana juice enzymatic browning by the addition of maltosyl-beta-cyclodextrin.

作者信息

López-Nicolás José M, Pérez-López Antonio J, Carbonell-Barrachina Angel, García-Carmona Francisco

机构信息

Department of Biochemistry and Molecular Biology-A, Faculty of Biology, University of Murcia, Campus de Espinardo, 30071 Murcia, Spain.

出版信息

J Agric Food Chem. 2007 Nov 14;55(23):9655-62. doi: 10.1021/jf0713399. Epub 2007 Oct 12.

Abstract

In recent years, the use of cyclodextrins (CDs) as antibrowning agents in fruit juices has received growning attention. However, there has been no detailed study of the behavior of these molecules as substances, which can lead to the darkening of foods. In this paper, when the color of fresh banana juice was evaluated in the presence of different CDs, the evolution of several color parameters was the opposite of that observed in other fruit juices. Moreover, a kinetic model based on the complexation by CDs of the natural browning inhibitors present in banana is developed for the first time to clarify the enzymatic browning activation of banana juice. Finally, the apparent complexation constant between the natural polyphenoloxidase inhibitors present in banana juice and maltosyl-beta-CD was calculated (Kci = 27.026 +/- 0.212 mM (-1)).

摘要

近年来,环糊精(CDs)作为果汁中的抗褐变剂受到了越来越多的关注。然而,对于这些分子作为可能导致食物变黑的物质的行为,尚未有详细的研究。在本文中,当在不同环糊精存在的情况下评估新鲜香蕉汁的颜色时,几个颜色参数的变化与在其他果汁中观察到的情况相反。此外,首次建立了一个基于环糊精与香蕉中天然褐变抑制剂络合作用的动力学模型,以阐明香蕉汁的酶促褐变活化过程。最后,计算了香蕉汁中天然多酚氧化酶抑制剂与麦芽糊精-β-环糊精之间的表观络合常数(Kci = 27.026 +/- 0.212 mM(-1))。

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