Jiang Shigui, Qiu Lihua, Zhou Falin, Huang Jianhua, Guo Yihui, Yang Keng
The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, People's Republic of China.
Mol Biol Rep. 2009 Jan;36(1):127-34. doi: 10.1007/s11033-007-9160-9. Epub 2007 Oct 13.
The techniques of homology cloning and anchored PCR were used to clone the Hsp90 gene from black tiger shrimp. The full length cDNA of black tiger shrimp Hsp90 (btsHsp90) contained a 5' untranslated region (UTR) of 72 bp, an ORF (open reading frame) of 2160 bp encoding a polypeptide of 720 amino acids with an estimated molecular mass of 83-kDa and a 3' UTR of 288 bp. The sequence of the coding region showed 90 and 84% homology with that of the Chiromantes haematocheir and Homo sapiens, respectively. Conserved signature sequences of Hsp90 gene family were found in the btsHsp90 deduced amino acid sequence. The temporal expressions of Hsp90 gene were constitutively in the black tiger shrimp tissues including liver, ovary, muscle, brain stomach, and heart, and their levels were markedly enhanced after 30-min heat treatment at 37 degrees C. In ovarian maturation stages, the expression of btsHsp90 was strongest in the second stage, weaker in the fourth and first stage.
采用同源克隆和锚定PCR技术从黑虎虾中克隆热休克蛋白90（Hsp90）基因。黑虎虾Hsp90（btsHsp90）的全长cDNA包含一个72 bp的5'非翻译区（UTR）、一个2160 bp的开放阅读框（ORF），编码一个由720个氨基酸组成的多肽，估计分子量为83 kDa，以及一个288 bp的3'UTR。编码区序列与中华绒螯蟹和人类的编码区序列分别具有90%和84%的同源性。在btsHsp90推导的氨基酸序列中发现了Hsp90基因家族的保守特征序列。Hsp90基因在黑虎虾的肝、卵巢、肌肉、脑、胃和心脏等组织中持续表达，在37℃热处理30分钟后其表达水平显著增强。在卵巢成熟阶段，btsHsp90在第二阶段表达最强，在第四阶段和第一阶段较弱。
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