一种用于快速检测人绒毛膜促性腺激素的免疫纳米金共振散射光谱探针。

An immunonanogold resonance scattering spectral probe for rapid assay of human chorionic gonadotrophin.

作者信息

Jiang Zhi-Liang, Zou Ming-Jing, Liang Ai-Hui

机构信息

School of Environment and Resource, Guangxi Normal University, Guilin 541004, China.

出版信息

Clin Chim Acta. 2008 Jan;387(1-2):24-30. doi: 10.1016/j.cca.2007.08.017. Epub 2007 Sep 7.

DOI:10.1016/j.cca.2007.08.017

PMID:17936256

Abstract

BACKGROUND

Several assays for human chorionic gonadotrophin (hCG) such as radioimmunoassay and fluorescence immunoassays were reported. So far, there was no report about the immunonanogold resonance scattering spectral (ING-RSS) assay based on the immunoreaction and resonance scattering (RS) effect.

METHODS

Three ING-RSS probes for hCG were prepared, using nanogold in size of 8, 10, 13 nm to label rabbit hCG antiserum (anti-hCG).

RESULTS

In citric acid-Na(2)HPO(4) buffer solution and in the presence of polyethylene glycol (PEG)6000, the immunoreaction between hCG and nanogold-labeled anti-hCG took place, the immunonanogold-complex was formed and deposited. The decreased intensity DeltaI(RS) for the nanogold in sizes of 8, 10, 13 nm was proportional to hCG concentration in the ranges of 40-10000, 40-10000 and 40-8000 mIU/ml, with the detection limits of 19.4, 15.1 and 13.6 mIU/ml, respectively. The hCG in urine samples were assayed by the ING-RSS assay, and by immunospectrophotometry. The results of both assays showed a close correlation.

CONCLUSION

This assay has simplicity, sensitivity and good selectivity for quantitative determination of hCG.

摘要

背景

已报道了几种人绒毛膜促性腺激素（hCG）检测方法，如放射免疫分析和荧光免疫分析。到目前为止，尚无基于免疫反应和共振散射（RS）效应的免疫纳米金共振散射光谱（ING-RSS）检测方法的报道。

方法

制备了三种用于hCG的ING-RSS探针，使用8、10、13nm大小的纳米金标记兔抗hCG血清（抗hCG）。

结果

在柠檬酸-Na₂HPO₄缓冲溶液中且存在聚乙二醇（PEG）6000的情况下，hCG与纳米金标记的抗hCG发生免疫反应，形成免疫纳米金复合物并沉淀。8、10、13nm大小的纳米金的共振散射强度降低值ΔI(RS)在40-10000、40-10000和40-8000mIU/ml范围内与hCG浓度成正比，检测限分别为19.4、15.1和13.6mIU/ml。采用ING-RSS检测法和免疫分光光度法对尿样中的hCG进行检测。两种检测方法的结果显示出密切相关性。