野猪（Sus scrofa）淋巴细胞功能相关抗原-1（CD11a/CD18）受体：cDNA测序、结构分析及与同源物的比较。

The wild boar (Sus scrofa) lymphocyte function-associated antigen-1 (CD11a/CD18) receptor: cDNA sequencing, structure analysis and comparison with homologues.

作者信息

Bergh Philippe G A C Vanden, Zecchinon Laurent L M, Fett Thomas, Desmecht Daniel J M

机构信息

Pathology Department, Faculty of Veterinary Medicine, University of Liege, Colonster Boulevard 20 B43, B-4000 Liege, Belgium.

出版信息

BMC Vet Res. 2007 Oct 15;3:27. doi: 10.1186/1746-6148-3-27.

DOI:10.1186/1746-6148-3-27

PMID:17937788

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2151945/

Abstract

BACKGROUND

The most predominant beta2-integrin lymphocyte function-associated antigen-1 (LFA-1, CD11a/CD18, alphaLbeta2), expressed on all leukocytes, is essential for many adhesive functions of the immune system. Interestingly, RTX toxin-producing bacteria specifically target this leukocyte beta2-integrin which exacerbates lesions and disease development.

RESULTS

This study reports the sequencing of the wild boar beta2-integrin CD11a and CD18 cDNAs. Predicted CD11a and CD18 subunits share all the main structural characteristics of their mammalian homologues, with a larger interspecies conservation for the CD18 than the CD11a. Besides these strong overall similarities, wild boar and domestic pig LFA-1 differ by 2 (CD18) and 1 or 3 (CD11a) substitutions, of which one is located in the crucial I-domain (CD11a, E168D).

CONCLUSION

As most wild boars are seropositive to the RTX toxin-producing bacterium Actinobacillus pleuropneumoniae and because they have sustained continuous natural selection, future studies addressing the functional impact of these polymorphisms could bring interesting new information on the physiopathology of Actinobacillus pleuropneumoniae-associated pneumonia in domestic pigs.

摘要

背景

最主要的β2整合素淋巴细胞功能相关抗原-1（LFA-1，CD11a/CD18，αLβ2）表达于所有白细胞，对免疫系统的许多黏附功能至关重要。有趣的是，产RTX毒素的细菌特异性靶向这种白细胞β2整合素，从而加剧病变和疾病发展。

结果

本研究报告了野猪β2整合素CD11a和CD18 cDNA的测序结果。预测的CD11a和CD18亚基具有其哺乳动物同源物的所有主要结构特征，CD18的种间保守性比CD11a更大。除了这些强烈的总体相似性外，野猪和家猪的LFA-1在2个位点（CD18）以及1个或3个位点（CD11a）存在差异，其中一个位于关键的I结构域（CD11a，E168D）。