Seierstad Therese, Røe Kathrine, Olsen Dag Rune
Department of Medical Physics, Rikshospitalet-Radiumhospitalet Medical Center, Oslo, Norway.
Radiother Oncol. 2007 Nov;85(2):187-94. doi: 10.1016/j.radonc.2007.09.009. Epub 2007 Oct 15.
To examine whether in vivo proton magnetic resonance spectroscopy ((1)H MRS) and diffusion-weighted magnetic resonance imaging (DW-MRI) can monitor radiation-induced changes in HT29 xenografts in mice.
HT29 xenografts in mice received a dose of 15Gy. In vivo(1)H MRS and DW-MRI were acquired pretreatment and 1, 3, 6 and 10 days post-irradiation. After imaging, tumors were excised for histological analysis. The amounts of necrosis, fibrosis and viable cells in the cross sections were scored and compared to changes in apparent diffusion coefficient (ADC) and choline/water ratio.
Radiation-induced necrosis in the xenografts was observed as increased tumor ADC. In-growth of fibrosis three days post-irradiation restricting water mobility was accompanied by decreased tumor ADC. Choline/water ratio correlated with metabolic activity and tumor growth.
ADC and choline/water ratio assessed by in vivo DW-MRI and (1)H MRS depicts radiation-induced changes in HT29 xenografts following irradiation.
研究体内质子磁共振波谱（(1)H MRS）和扩散加权磁共振成像（DW-MRI）能否监测小鼠HT29异种移植瘤的辐射诱导变化。
给小鼠的HT29异种移植瘤给予15Gy剂量的辐射。在照射前以及照射后1、3、6和10天进行体内(1)H MRS和DW-MRI检查。成像后，切除肿瘤进行组织学分析。对横截面中的坏死、纤维化和存活细胞数量进行评分，并与表观扩散系数（ADC）和胆碱/水比率的变化进行比较。
异种移植瘤中辐射诱导的坏死表现为肿瘤ADC增加。照射后三天纤维化的向内生长限制了水的流动性，同时肿瘤ADC降低。胆碱/水比率与代谢活性和肿瘤生长相关。
通过体内DW-MRI和(1)H MRS评估的ADC和胆碱/水比率描绘了照射后HT29异种移植瘤的辐射诱导变化。
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