Rammouz Georges, Lacroix Marlène, Garrigues Jean Christophe, Poinsot Véréna, Couderc François
Université Paul Sabatier, IMRCP, UMR 5623, Toulouse, France.
Biomed Chromatogr. 2007 Dec;21(12):1223-39. doi: 10.1002/bmc.893.
This paper reviews analytical methods, instrumental developments and applications for derivatization of primary amines with naphthalene-2,3-dicarboxaldehyde using fluorescence and chemiluminescence detection with capillary electrophoresis (CE) and high performance liquid chromatography (HPLC). The use of lasers as well as lamps as the excitation source for fluorescence detection is discussed. The detection limit observed with naphthalene-2,3-dicarboxaldehyde derivatization is often lower and better than those obtained with other analytical separations and other fluorescent dyes. In addition, this paper describes the crucial points that influence the stability of NDA primary amine derivatives, and summarize the separation, derivatization and migration conditions of the different techniques, with their advantages and drawbacks.
本文综述了使用萘-2,3-二甲醛对伯胺进行衍生化,并结合毛细管电泳(CE)和高效液相色谱(HPLC)的荧光和化学发光检测的分析方法、仪器发展及应用。讨论了使用激光以及灯作为荧光检测的激发源。萘-2,3-二甲醛衍生化所观察到的检测限通常比其他分析分离方法和其他荧光染料所获得的检测限更低且更好。此外,本文描述了影响NDA伯胺衍生物稳定性的关键点,并总结了不同技术的分离、衍生化和迁移条件及其优缺点。