Harper Lisa J, Piper Kim, Common John, Fortune Farida, Mackenzie Ian C
Institute of Cell and Molecular Science, Queen Mary's School of Medicine and Dentistry, Whitechapel, London, UK.
J Oral Pathol Med. 2007 Nov;36(10):594-603. doi: 10.1111/j.1600-0714.2007.00617.x.
The initiation, growth, recurrence and metastasis of solid tumours, including squamous cell carcinoma of the head and neck region, have been related to the behaviour of a small subpopulation of 'tumour-initiating' cells. Cells with stem cell characteristics have also been identified in cell lines derived from cancers and the aim of the present work was to extend examination of such cells. Established cell lines were examined for their patterns of colony morphologies and staining, the presence of a Hoechst dye-excluding 'side population', expression of the putative stem cell markers CD44, CD133 and CD29, and their ability to grow as 'cancer spheroids'. Two cell lines, CaLH2 and CaLH3, recently generated from HNSCC tumour biopsies, were similarly examined. All cell lines showed a holoclone/meroclone/paraclone series of colony morphologies and cell sorting indicated that CD44 marker expression was related to clonogenicity. FACS analysis after exposure to Hoechst dye indicated that the CA1, H357 and UK1 cell lines contain a dye-excluding 'side population', a property associated with stem-like subpopulations. When held in suspension, all cell lines formed spheroids that could be re-passaged. These observations indicate that cell lines derived from HNSCC contain cells with stem cell properties and that such cell lines may provide experimental systems relevant to the behaviour of stem cells present in the tumours of origin and to their responses to therapy.
实体瘤的起始、生长、复发和转移，包括头颈部区域的鳞状细胞癌，都与一小部分“肿瘤起始”细胞的行为有关。在源自癌症的细胞系中也鉴定出了具有干细胞特征的细胞，本研究的目的是扩展对此类细胞的研究。对已建立的细胞系进行了集落形态和染色模式、Hoechst染料排除“侧群”的存在、假定干细胞标志物CD44、CD133和CD29的表达以及它们作为“癌球”生长的能力的检测。对最近从头颈部鳞状细胞癌肿瘤活检中产生的两个细胞系CaLH2和CaLH3进行了类似的检测。所有细胞系均显示出全克隆/部分克隆/旁克隆系列的集落形态，细胞分选表明CD44标志物表达与克隆形成能力有关。用Hoechst染料处理后的流式细胞术分析表明，CA1、H357和UK1细胞系含有染料排除“侧群”，这一特性与干细胞样亚群相关。当悬浮培养时，所有细胞系都形成了可以再次传代的球体。这些观察结果表明，源自头颈部鳞状细胞癌的细胞系含有具有干细胞特性的细胞，并且此类细胞系可能提供与原发肿瘤中存在的干细胞行为及其对治疗的反应相关的实验系统。
