水凝胶中人类胚胎生殖细胞衍生细胞的软骨形成分化

Chondrogenic differentiation of human embryonic germ cell derived cells in hydrogels.

作者信息

Varghese Shyni, Theprungsirikul Paranduangji, Ferran Angela, Hwang Nathaniel, Canver Adam, Elisseeff Jennifer

出版信息

Conf Proc IEEE Eng Med Biol Soc. 2006;2006:2643-6. doi: 10.1109/IEMBS.2006.259710.

DOI:10.1109/IEMBS.2006.259710

Abstract

Human embryonic germ (hEG) cells have the potential to self-renew over long periods of time and differentiate into various lineages. Cells derived from embryoid bodies of hEG cells express a broad spectrum of gene markers and have been induced towards cells of ecto-dermal and recently endo-dermal and mesenchymal lineages. LVEC cells express a number of surface marker proteins characteristic of mesenchymal stem cells (MSCs), indicating the potential of these cells to differentiate into mesenchymal tissues. Here we demonstrate the homogenous differentiation of LVEC cells into hyaline cartilage. Three dimensional tissue formation is achieved by encapsulating cells in synthetic hydrogels followed by incubation in chondrocyte-conditioned culture medium. Homogenous hyaline cartilage was produced, even after 63 population doublings (13 passages). The high proliferative capacity of these cells without teratoma formation, homogenous differentiation, and three-dimensional cartilage tissue formation suggests the significant potential of LVEC cells for cartilage tissue engineering applications.

摘要

人胚胎生殖（hEG）细胞有长期自我更新并分化为各种谱系细胞的潜力。源自hEG细胞胚状体的细胞表达多种基因标志物，并已被诱导分化为外胚层细胞，最近还可分化为内胚层和间充质谱系细胞。LVEC细胞表达一些间充质干细胞（MSC）特有的表面标志物蛋白，表明这些细胞有分化为间充质组织的潜力。在此，我们展示了LVEC细胞向透明软骨的同质分化。通过将细胞包裹在合成水凝胶中，然后在软骨细胞条件培养基中培养来实现三维组织形成。即使经过63次群体倍增（13代），仍能产生同质的透明软骨。这些细胞的高增殖能力、无畸胎瘤形成、同质分化以及三维软骨组织形成表明LVEC细胞在软骨组织工程应用中具有巨大潜力。