Murex第四代HIV酶免疫测定法在坦桑尼亚青少年中的低特异性
Low specificity of the Murex fourth-generation HIV enzyme immunoassay in Tanzanian adolescents.
作者信息
Everett Dean B, Weiss Helen A, Changalucha John, Anemona Alessandra, Chirwa Tobias, Ross David A, Watson-Jones Deborah, Parry John V, Hayes Richard, Mabey David C
机构信息
London School of Hygiene & Tropical Medicine, London, UK.
出版信息
Trop Med Int Health. 2007 Nov;12(11):1323-6. doi: 10.1111/j.1365-3156.2007.01933.x. Epub 2007 Oct 22.
OBJECTIVE
To determine the specificity of the Abbott Murex HIV antigen/antibody combination enzyme immunoassay (EIA) for the diagnosis of HIV infection in Tanzania.
METHODS
A cross-sectional survey of 7333 Tanzanian adolescents and young adults was carried out. Sera testing positive by the Murex assay were further evaluated using a battery of other EIA which detect either antibody to HIV-1 or p24 antigen, and by PCR using pol primers.
RESULTS
Of the 674 sera testing positive by the Murex assay, only 53 (7.9%) were confirmed. The specificity of the Murex assay was 91.5%.
CONCLUSIONS
Serological tests for HIV may perform differently in different populations. New diagnostic tests should not be introduced into populations in which they have not been evaluated.
目的
确定雅培Murex HIV抗原/抗体联合酶免疫测定法（EIA）在坦桑尼亚诊断HIV感染的特异性。
方法
对7333名坦桑尼亚青少年和青年进行了横断面调查。通过Murex检测呈阳性的血清，使用一系列其他检测HIV-1抗体或p24抗原的酶免疫测定法以及使用pol引物的聚合酶链反应（PCR）进行进一步评估。
结果
在通过Murex检测呈阳性的674份血清中，仅53份（7.9%）得到确诊。Murex检测的特异性为91.5%。
结论
HIV血清学检测在不同人群中可能表现不同。新的诊断检测不应引入未对其进行评估的人群。
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