[Study on the effect of iodine excess on cell apoptosis in rat thyroid cells (FRTL)].

OBJECTIVE

To explore the mechanism of iodine over-treatment to FRTL cell on the cell apoptosis through observing the cell apoptosis related gene.

METHODS

The cells were treated with potassium iodide (0, 5, 10, 50 and 100mmol/L). After treatment 24 hours, the cells were collected and analyzed by flow cytometry. The mRNA levels of fas, fasL, Bcl-2 and Bax of 50mmol/L and 100mmol/L groups were determined with real-time PCR.

RESULTS

Compared with the normal control group, the rates of cell apoptosis of 10, 50 and 100mmol/L iodine groups increased significantly. Compared with the normal control group, the mRNA levels of fasL and Bax in 10 and 50mmol/L iodine groups increased significantly while the level of Bcl-2 decreased significantly. There was no significant difference fas mRNA levels between the normal control group and 10mmol/L iodine group, but the fas mRNA level in 50mmol/L iodine group increased significantly.

CONCLUSION

The excessive iodine treatment increased the apoptosis of FRTL cells which might be related to gene expressions of fas, fasL, Bcl-2 and Bax.