De Giovanni C, Nicoletti G, Galli M C, Nanni P
Istituto di Cancerologia dell'Università di Bologna.
Boll Soc Ital Biol Sper. 1979 Jan 30;55(2):117-21.
A method for determining cytoplasmic progesterone receptor was standardized in normal human endometrium comparing two different tracers, 3H-progesterone (3H-P) and 3H-medroxyprogesterone acetate (3H-MAP), a synthetic progestin which does not bind to Corticosteroid Binding Globulin (CBG). Receptor assays were performed as previously reported for 17beta-estradiol receptor, with slight modifications: incubation lasted 1 hr at 0 degree C, followed by 5 min DCC exposure under the same conditions. When 3H-P was employed as tracer, blanks performed with cold MAP gave similar results as using cortisol in incubation tubes and progesterone and cortisol in blanks. 3H-MAP was a good tracer for progesterone receptor because it neither bound to CBG nor to androgen or cortisol receptors; it had very high affinity and specificity for P-R; it was not metabolized by cytosol at 0 degree C and, finally, it detected receptor amounts quite comparable to those obtained using 3H-P.
一种测定人正常子宫内膜细胞质孕酮受体的方法得以标准化，该方法比较了两种不同的示踪剂：³H-孕酮（³H-P）和³H-醋酸甲羟孕酮（³H-MAP），³H-MAP是一种不与皮质类固醇结合球蛋白（CBG）结合的合成孕激素。受体测定按照先前报道的17β-雌二醇受体的方法进行，稍有改动：在0℃下孵育1小时，然后在相同条件下进行5分钟的葡聚糖包被活性炭（DCC）处理。当使用³H-P作为示踪剂时，用冷的MAP进行的空白试验与在孵育管中使用皮质醇以及在空白中使用孕酮和皮质醇所得到的结果相似。³H-MAP是孕酮受体的良好示踪剂，因为它既不与CBG结合，也不与雄激素或皮质醇受体结合；它对孕酮受体具有非常高的亲和力和特异性；在0℃下它不会被胞质溶胶代谢，最后，它检测到的受体量与使用³H-P所获得的受体量相当。
