从北欧鳕鱼（Gadus morhua L.）中分离出的鳗弧菌菌株的表型、血清学和基因特征分析

Phenotypic, serological and genetic characterization of Pseudomonas anguilliseptica strains isolated from cod, Gadus morhua L., in northern Europe.

作者信息

Balboa S, Ferguson H W, Romalde J L

机构信息

Departamento de Microbiología y Parasitología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela, Spain.

出版信息

J Fish Dis. 2007 Nov;30(11):657-64. doi: 10.1111/j.1365-2761.2007.00849.x.

DOI:10.1111/j.1365-2761.2007.00849.x

PMID:17958609

Abstract

The biochemical, serological and genetic characteristics of six strains of Pseudomonas anguilliseptica isolated from cod, Gadus morhua, in Scotland were compared to well characterized isolates of this same bacterial species but of different origin. Biochemical and physiological analyses showed that this group of isolates was highly homogeneous, their characteristics matching previous descriptions of the pathogen. Similar results were obtained for the six cod isolates in the serological assays, all of them belonging to the serotype O1. Marked homogeneity was observed also in the genetic study, analysed by means of RAPD, ERIC-PCR and REP-PCR procedures, showing that they were similar to isolates from gilthead seabream, Sparus aurata, black spot seabream, Pagellus bogaraveo, and turbot, Psetta maxima. Virulence assays demonstrated that the cod isolates were highly pathogenic for turbot and sole, Solea senegalensis, with LD50 between 7.6 x 10(4) and 5 x 10(7) bacterial cells per fish.

摘要

将从苏格兰的鳕鱼（Gadus morhua）中分离出的6株鳗弧菌（Pseudomonas anguilliseptica）的生化、血清学和遗传特征，与已充分表征的、但来源不同的同一细菌物种的分离株进行了比较。生化和生理分析表明，这组分离株高度同源，其特征与该病原体先前的描述相符。在血清学检测中，这6株鳕鱼分离株也得到了类似结果，它们均属于O1血清型。在通过RAPD、ERIC-PCR和REP-PCR程序进行分析的遗传研究中，也观察到了显著的同源性，表明它们与来自金头鲷（Sparus aurata）、黑鲷（Pagellus bogaraveo）和大菱鲆（Psetta maxima）的分离株相似。毒力试验表明，鳕鱼分离株对大菱鲆和塞内加尔鳎（Solea senegalensis）具有高度致病性，每条鱼的半数致死剂量（LD50）在7.6×10⁴至5×10⁷个细菌细胞之间。