[利用微卫星多态性检测完全性葡萄胎亲本来源的研究]
[Study on parental origin of complete hydatidiform mole detected by polymorphisms of microsatellite].
作者信息
Deng Shun-sheng, Ding Zhi-ming, Qian Jian-hua, Lü Wei-guo, Wan Xiao-yun, Xie Xing
机构信息
Department of Gynecological Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
出版信息
Zhonghua Fu Chan Ke Za Zhi. 2007 Jul;42(7):468-71.
OBJECTIVE
Through detection of 9 loci of polymorphisms of microsatellite to investigate the parental origin of complete hydatidiform mole.
METHODS
Using the technology and method of multipolymerase chain reaction and electrophoresis in denatured polyacrylamide gel and silver stain detection, we carried out DNA analysis on 50 cases of complete hydatidiform mole diagnosed by histopathology and 50 copies of the couples' peripheral blood.
RESULTS
There are 7 cases of biparental complete hydatidiform mole (14%); and 43 cases of androgenetic complete hydatidiform mole (86%) among 50 cases of complete hydatidiform mole.
CONCLUSIONS
Detection of polymorphisms of microsatellite is a technology that can be used to identify the parental origin of complete hydatidiform mole. It is simple, quick, reliable and highly efficient.
目的
通过检测微卫星多态性的9个位点，探讨完全性葡萄胎的亲代来源。
方法
采用多重聚合酶链反应技术及变性聚丙烯酰胺凝胶电泳和银染检测方法，对50例经组织病理学诊断的完全性葡萄胎及50份配偶外周血进行DNA分析。
结果
50例完全性葡萄胎中，有7例为双亲来源的完全性葡萄胎（14%）；43例为孤雄来源的完全性葡萄胎（86%）。
结论
微卫星多态性检测是一种可用于鉴定完全性葡萄胎亲代来源的技术。该技术简单、快速、可靠且高效。
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