Chodaparambil Jayanth V, Barbera Andrew J, Lu Xu, Kaye Kenneth M, Hansen Jeffrey C, Luger Karolin
Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523-1870, USA.
Nat Struct Mol Biol. 2007 Nov;14(11):1105-7. doi: 10.1038/nsmb1334. Epub 2007 Oct 28.
Local nucleosome-nucleosome interactions in cis drive chromatin folding, whereas interactions in trans lead to fiber-fiber oligomerization. Here we show that peptides derived from the histone H4 tail and Kaposi's sarcoma herpesvirus LANA protein can replace the endogenous H4 tail, resulting in array folding and oligomerization. Neutralization of a LANA binding site on the histone surface enhanced rather than abolished nucleosome-nucleosome interactions. We maintain that the contoured nucleosome surface is centrally involved in regulating chromatin condensation.
顺式作用下的局部核小体-核小体相互作用驱动染色质折叠,而反式作用下的相互作用则导致纤维-纤维寡聚化。在此,我们表明,源自组蛋白H4尾部和卡波西肉瘤疱疹病毒LANA蛋白的肽段可取代内源性H4尾部,从而导致阵列折叠和寡聚化。组蛋白表面LANA结合位点的中和增强而非消除了核小体-核小体相互作用。我们认为,具轮廓的核小体表面在调节染色质凝聚中起核心作用。