Sliwowska Izabela, Kopczyński Zygmunt
Zakładu Diagnostyki Laboratoryjnej Katedry Onkologii Uniwersytetu Medycznego im. Karola Marcinkowskiego w Poznaniu.
Wiad Lek. 2007;60(5-6):241-7.
Zymography is an electrophoretic method for measuring proteolytic activity. The method is based on polyacrylamide gels impregnated with a protein substrate (gelatin, casein), which is degraded by the proteases. It is already widely used for research on extracellular matrix degrading enzymes, in particular the matrix metalloproteinases (MMPs). The aim of this study was to evaluate the zymography used to estimate gelatinase A (pro-MMP-2, 72 kDa) and gelatinase B (pro-MMP-9, 92 kDa) in serum of women with breast cancer.
The study was conducted on the serum of 90 female with breast cancer aged 32-85 years (average 57.2 year) and in a group of 30 women with benign breast diseases aged 34-87 years (average 55.4 year).
The results showed significantly higher activity ofgelatinase A and gelatinase B in the group of women with breast cancer that in the control group. The activity of gelatinase A was over 0.48 AU/ml in 50 women (55.6%) and gelatinase B in 46 women in this group (51.1%). Further analysis showed a strong correlation between activity of gelatinase A and B and advance stage of breast cancer, lymph node status, and tumour burden. An elevated activity of gelatinases in patients with breast cancer confirms the role of these enzymes in the development of such tumours. There was no significant difference in the gelatinases activity between the serum samples from patients with breast cancer and those from women with benign breast diseases. The diagnostic sensitivity of this procedure used to estimate gelatinase A and gelatinase B activity carried out 56% and 51%, and the diagnostic specificity 75% and 77%, respectively.
Zymography is a simple, sensitive, and functional assay for analysing proteolytic activity of gelatinase A and gelatinase B. The diagnostic accuracy of gelatinases evaluation is limited by the lack of sensitivity and specificity in early stage of the disease and in differentiation of breast cancer from benign diseases. Measuring their activity in serum breast cancer patients indicates the patient's clinical condition. A good correlation between serum activity of gelatinases and the prognostic factors suggest its usefulness as a marker both in the follow-up and in the prognosis of breast cancer patients.
酶谱法是一种用于测量蛋白水解活性的电泳方法。该方法基于浸渍有蛋白质底物（明胶、酪蛋白）的聚丙烯酰胺凝胶，蛋白酶可使其降解。它已广泛用于细胞外基质降解酶的研究，尤其是基质金属蛋白酶（MMPs）。本研究的目的是评估用于估计乳腺癌女性血清中明胶酶A（前MMP-2，72 kDa）和明胶酶B（前MMP-9，92 kDa）的酶谱法。
本研究对90名年龄在32 - 85岁（平均57.2岁）的乳腺癌女性血清以及30名年龄在34 - 87岁（平均55.4岁）的乳腺良性疾病女性血清进行了检测。
结果显示，乳腺癌女性组中明胶酶A和明胶酶B的活性显著高于对照组。该组中有50名女性（55.6%）的明胶酶A活性超过0.48 AU/ml，46名女性（51.1%）的明胶酶B活性超过此值。进一步分析表明，明胶酶A和B的活性与乳腺癌的进展期、淋巴结状态及肿瘤负荷之间存在强相关性。乳腺癌患者中明胶酶活性升高证实了这些酶在这类肿瘤发生发展中的作用。乳腺癌患者血清样本与乳腺良性疾病女性血清样本中的明胶酶活性无显著差异。用于估计明胶酶A和B活性的该检测方法的诊断敏感性分别为56%和51%，诊断特异性分别为75%和77%。
酶谱法是一种用于分析明胶酶A和明胶酶B蛋白水解活性的简单、灵敏且实用的检测方法。明胶酶评估的诊断准确性受到疾病早期缺乏敏感性和特异性以及难以区分乳腺癌与良性疾病的限制。检测乳腺癌患者血清中的明胶酶活性可表明患者的临床状况。血清明胶酶活性与预后因素之间的良好相关性表明其在乳腺癌患者随访和预后评估中作为标志物具有实用性。
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