Ternovskiĭ V I, Chernokhvostov Iu V, Fomkina M G, Montrel' M M
Biofizika. 2007 Sep-Oct;52(5):825-9.
A potentiometric biosensor has been designed on the basis of glass pH-electrode with a sensing device of the microcellular polyelectrolytic coating containing urease. The polymeric walls of the coating are readily permeable for low-molecular weight compounds, including urea, but are impermeable for macromolecules. The main characteristics of the biosensor in various experimental solutions containing urea, low-molecular-weight salt, and buffer have been obtained. The sensor has been shown to be stable for at least three weeks. The standard curves of the sensor are linear in the range of urea concentrations from 0.2 to 20 mM.
基于带有含脲酶的微孔聚电解质涂层传感装置的玻璃pH电极，设计了一种电位型生物传感器。该涂层的聚合物壁对包括尿素在内的低分子量化合物具有良好的渗透性，但对大分子是不可渗透的。已获得该生物传感器在含有尿素、低分子量盐和缓冲液的各种实验溶液中的主要特性。结果表明，该传感器至少可稳定三周。该传感器的标准曲线在尿素浓度为0.2至20 mM的范围内呈线性。
Zotero 插件