白细胞介素-11减轻异环磷酰胺诱导的出血性膀胱炎。
Interleukin-11 attenuates ifosfamide-induced hemorrhagic cystitis.
作者信息
Mota Jose M, Brito Gerly A, Loiola Raphael T, Cunha Fernando Q, Ribeiro Ronaldo de A
机构信息
Department of Physiology, School of Medicine, Federal University of Ceara, Fortaleza, Brazil.
出版信息
Int Braz J Urol. 2007 Sep-Oct;33(5):704-10. doi: 10.1590/s1677-55382007000500013.
OBJECTIVE
To investigate the possible protective effect of recombinant human interleukin-11 (rhIL-11) against ifosfamide (IFS)-induced hemorrhagic cystitis (HC).
MATERIALS AND METHODS
Male Swiss mice (20-30g) were pretreated with rhIL-11 (25-625 mg, subcutaneously.) 30 min before intraperitoneal injection of IFS (400 mg/kg) or with saline (control group). Twelve hours later, HC was evaluated by bladder wet weight (BWW) to quantify edema, Evans blue extravasation (EBE) to measure vascular permeability, and macroscopic and microscopic analysis. All bladders were assessed by histopathological analysis.
RESULTS
rhIL-11 (at 125 and 625 mg) attenuated the IFS- induced increase of BWW (37.48% and 45.44%, respectively, p < 0.05) and EBE (62.35% and 56.47%, respectively, p < 0.05). IFS- induced macroscopic edema and hemorrhage and microscopic alterations, were also prevented by rhIL-11 at 625 microg. (p < 0.05).
CONCLUSION
Our results demonstrate a protective effect of rhIL-11 on experimental IFS- induced HC, not previously reported.
目的
研究重组人白细胞介素-11（rhIL-11）对异环磷酰胺（IFS）诱导的出血性膀胱炎（HC）的可能保护作用。
材料与方法
雄性瑞士小鼠（20 - 30克）在腹腔注射IFS（400毫克/千克）前30分钟皮下注射rhIL-11（25 - 625毫克）或生理盐水（对照组）。12小时后，通过膀胱湿重（BWW）评估HC以量化水肿，通过伊文思蓝外渗（EBE）测量血管通透性，并进行宏观和微观分析。所有膀胱均通过组织病理学分析进行评估。
结果
rhIL-11（125毫克和625毫克）减轻了IFS诱导的BWW增加（分别为37.48%和45.44%，p < 0.05）和EBE增加（分别为62.35%和56.47%，p < 0.05）。625微克的rhIL-11还预防了IFS诱导的宏观水肿、出血和微观改变（p < 0.05）。
结论
我们的结果证明了rhIL-11对实验性IFS诱导的HC具有保护作用，这是以前未报道过的。
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