Tani Akio, Somyoonsap Peechapack, Minami Toshiyuki, Kimbara Kazuhide, Kawai Fusako
Research Institute for Bioresources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046, Japan.
Arch Microbiol. 2008 Apr;189(4):407-10. doi: 10.1007/s00203-007-0320-z. Epub 2007 Nov 6.
Sphingopyxis macrogoltabida strain 103 possesses polyethylene-glycol (PEG)-inducible pegBCDAE operon encoding the genes relevant to PEG degradation. PEG is converted to PEG-carboxylate by PegA (PEG dehydrogenase) and PegC (PEG-aldehyde dehydrogenase). In this study, the recombinant PegE (homologous to acyl-CoA synthetases) was characterized. PegE was an acyl-CoA synthetase active for PEG-carboxylate and fatty acids. Judging from the nature of this kind of protein (located on the cytoplasmic membrane as a translocator), PegE might be responsible for the translocation of PEG-carboxylate from the periplasm into the cytoplasm or for the detoxification of strong acidity of the substrate.
嗜麦芽窄食单胞菌菌株103拥有聚乙二醇（PEG）诱导型pegBCDAE操纵子，该操纵子编码与PEG降解相关的基因。PEG被PegA（PEG脱氢酶）和PegC（PEG醛脱氢酶）转化为PEG羧酸盐。在本研究中，对重组PegE（与酰基辅酶A合成酶同源）进行了表征。PegE是一种对PEG羧酸盐和脂肪酸具有活性的酰基辅酶A合成酶。从这类蛋白质的性质（作为转运体位于细胞质膜上）判断，PegE可能负责将PEG羧酸盐从周质转运到细胞质中，或者负责底物强酸性的解毒。
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