Lee Hyeon Ii, Kim Mee Kum, Oh Joo Youn, Ko Jung Hwa, Lee Hyun Ju, Wee Won Ryang, Lee Jin Hak
Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Korea.
Xenotransplantation. 2007 Nov;14(6):612-8. doi: 10.1111/j.1399-3089.2007.00433.x.
To investigate alpha Gal (Gal alpha1-3Gal beta 1-4GlcNAc-R) expression of the porcine cornea in vitro, in vivo and after xenotransplantation.
Using the GS-IB4 lectin (Griffonia simplifolia I isolectin B4), the expression of alpha Gal was evaluated in the normal porcine cornea and in cultured corneal stromal and endothelial cells of the second passages. The distribution of alpha Gal epitopes was also evaluated in corneal grafts at 4, 7 and 10 days after pig-to-rat orthotopic corneal transplantation.
The expression of alpha Gal was mostly confined to the anterior stromal keratocytes of the normal porcine cornea. However, reactivity for GS-IB4 was markedly increased during cell culture passage (P)-by 18.0% (P1) and 39.0% (P2) in cultivated keratocytes, and by 62.1% (P1) and 87.1% (P2) in cultured endothelial cells. The expression of alpha Gal epitopes was gradually enhanced in all corneal layers of the graft after transplantation.
Alpha Gal expression in the cornea was gradually induced during in vitro culture and after xenotransplantation, suggesting a role of putative Gal-related acute humoral rejection in porcine corneal xenotransplantation.
研究猪角膜在体外、体内及异种移植后的α-半乳糖（Galα1-3Galβ1-4GlcNAc-R）表达情况。
使用GS-IB4凝集素（简叶豆凝集素I同工凝集素B4），评估正常猪角膜以及第二代培养的角膜基质细胞和内皮细胞中的α-半乳糖表达。还评估了猪到大鼠原位角膜移植后4天、7天和10天角膜移植物中α-半乳糖表位的分布。
α-半乳糖的表达主要局限于正常猪角膜的前基质角膜细胞。然而，在细胞培养传代（P）过程中，GS-IB4的反应性显著增加——在培养的角膜细胞中，P1时增加18.0%，P2时增加39.0%；在培养的内皮细胞中，P1时增加62.1%，P2时增加87.1%。移植后移植物的所有角膜层中α-半乳糖表位的表达逐渐增强。
角膜中的α-半乳糖表达在体外培养和异种移植后逐渐被诱导，提示假定的与半乳糖相关的急性体液排斥在猪角膜异种移植中起作用。
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