Dewey Colin N
Department of Electrical Engineering and Computer Sciences, University of California, Berkeley, USA.
Methods Mol Biol. 2007;395:221-36. doi: 10.1007/978-1-59745-514-5_14.
The availability of an increasing number of whole genome sequences presents us with the need for tools to quickly put them into a nucleotide-level multiple alignment. Mercator and MAVID are two programs that can be combined to accomplish this task. Given multiple whole genomes as input, Mercator is first used to construct an orthology map, which is then used to guide nucleotide-level multiple alignments produced by MAVID. These programs are both fast and freely available, allowing researchers to perform genome alignments on a single laptop. This tutorial will guide the researcher through the steps required for whole-genome alignment with Mercator and MAVID.
越来越多全基因组序列的出现,使我们需要能够快速将它们进行核苷酸水平多重比对的工具。Mercator和MAVID是两个可结合起来完成这项任务的程序。以多个全基因组作为输入,首先使用Mercator构建一个直系同源图谱,然后用该图谱来指导由MAVID生成的核苷酸水平多重比对。这些程序既快速又免费,研究人员可以在一台笔记本电脑上进行基因组比对。本教程将指导研究人员完成使用Mercator和MAVID进行全基因组比对所需的步骤。