Toda Kyoko, Chiba Kyoko, Ono Tomotada
National Inst. of Crop Science, 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan.
J Food Sci. 2007 Mar;72(2):C108-13. doi: 10.1111/j.1750-3841.2006.00248.x.
The physicochemical properties of soymilk and the texture of tofu were compared with regard to 2 kinds of soymilk, one of which was prepared by squeezing homogenates before heating and the other was prepared by squeezing after heating raw soymilk with okara, residue of soymilk production. Relative particulate protein content and viscosity were higher and pH was lower in the soymilk prepared by the latter method, in which liberated lipid bodies were decreased and more lipids were precipitated with protein after centrifugation, suggesting a change in the interaction between proteins and lipids. A difference in the distribution of proteins and lipids was also implied by analysis with a laser particle size analyzer. The breaking stress of tofu made with 0.30% glucono-delta-lactone increased in accordance with an increase in particulate protein. The calcium and magnesium contents increased in soymilk prepared by squeezing after heating with okara. Viscosity was slightly increased and pH decreased by adding calcium to the soymilk, but the particulate protein content and breaking stress of tofu did not increase significantly. To examine the effect of macromolecules, okara was extracted by boiling and dialyzed. Viscosity and particulate protein content in soymilk increased as the dialyzed extracts of the okara were added. The breaking stress of tofu was increased by adding the dialyzed extracts but excessive amounts of the extracts resulted in softer tofu. Spectra of Fourier-transform infrared spectroscopy and electrophoresis-separated patterns of proteins indicated that the dialyzed extracts contained mainly polysaccharides and the Basic 7S globulin protein.
比较了两种豆浆的理化性质以及豆腐的质地,其中一种豆浆是在加热前对匀浆进行挤压制备的,另一种是在将生豆浆与豆渣(豆浆生产的残渣)一起加热后进行挤压制备的。后一种方法制备的豆浆中,相对颗粒蛋白含量和粘度较高,pH值较低,其中游离脂质体减少,离心后更多的脂质与蛋白质一起沉淀,这表明蛋白质和脂质之间的相互作用发生了变化。激光粒度分析仪分析也暗示了蛋白质和脂质分布的差异。用0.30%葡萄糖酸-δ-内酯制作的豆腐的断裂应力随着颗粒蛋白的增加而增加。在与豆渣一起加热后进行挤压制备的豆浆中,钙和镁的含量增加。向豆浆中添加钙会使粘度略有增加,pH值降低,但豆腐的颗粒蛋白含量和断裂应力没有显著增加。为了研究大分子的作用,将豆渣煮沸提取并透析。随着添加豆渣的透析提取物,豆浆中的粘度和颗粒蛋白含量增加。添加透析提取物会增加豆腐的断裂应力,但过量的提取物会使豆腐更软。傅里叶变换红外光谱和蛋白质电泳分离图谱表明,透析提取物主要含有多糖和碱性7S球蛋白。