Qi Zhi-Mei, Xia Shanhong, Matsuda Naoki
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100080, China.
Anal Biochem. 2008 Mar 1;374(1):196-202. doi: 10.1016/j.ab.2007.10.013. Epub 2007 Oct 14.
Evanescent wave has been recognized as a highly sensitive optical probe for surface monitoring. By use of slab optical waveguides, time-resolved evanescent wave absorption spectroscopy was developed for the investigation of the interfacial behavior of biomolecules with a chromophore. In this study, 30-microm thick glass sheets served as freestanding multimode waveguides that were combined with a simple fiber-coupling method to lead to a broadband evanescent wave absorption spectrometer. With such a homemade instrument, adsorption of heme proteins onto glass slides from static aqueous solution was monitored in situ. The experimental results reveal that the interfacial behavior of myoglobin (Mb) is different from that of hemoglobin (Hb) and cytochrome c (Cc). Formation of dynamic equilibrium for Mb adsorption at both hydrophilic and hydrophobic surfaces always is behind the occurrence of the maximum coverage. However, simultaneous formation of the dynamic equilibrium and the maximum coverage was observed for Hb and Cc adsorptions.
倏逝波已被公认为是一种用于表面监测的高灵敏度光学探针。通过使用平板光波导，开发了时间分辨倏逝波吸收光谱法，用于研究具有发色团的生物分子的界面行为。在本研究中，30微米厚的玻璃板用作独立的多模波导，通过一种简单的光纤耦合方法，制成了一台宽带倏逝波吸收光谱仪。使用这种自制仪器，原位监测了血红素蛋白从静态水溶液吸附到载玻片上的过程。实验结果表明，肌红蛋白（Mb）的界面行为不同于血红蛋白（Hb）和细胞色素c（Cc）。在亲水性和疏水性表面上，Mb吸附形成动态平衡总是发生在最大覆盖率出现之后。然而，对于Hb和Cc的吸附，观察到动态平衡和最大覆盖率同时形成。
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