Esrafili Ali, Yamini Yadollah, Shariati Shahab
Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran.
Anal Chim Acta. 2007 Dec 5;604(2):127-33. doi: 10.1016/j.aca.2007.10.012. Epub 2007 Oct 17.
The applicability of hollow fiber-based liquid phase microextraction (HF-LPME) was evaluated for the extraction and preconcentration of three antidepressant drugs (amitriptyline, imipramine and sertraline) prior to their determination by HPLC-UV. The target drugs were extracted from 11.0 mL of aqueous solution with pH 12.0 (source phase) into an organic extracting solvent (n-dodecane) impregnated in the pores of a hollow fiber and finally back extracted into 24 microL of aqueous solution located inside the lumen of the hollow fiber and adjusted to pH 2.1 using 0.1M of H3PO4 (receiving phase). The extraction was performed due to pH gradient between the inside and outside of the hollow fiber membrane. In order to obtain high extraction efficiency, the parameters affecting the HF-LPME including pH of the source and receiving phases, the type of organic phase, ionic strength and volume of the source phase, stirring rate and extraction time were studied and optimized. Under the optimized conditions, enrichment factors up to 300 were achieved and the relative standard deviation (R.S.D.%) of the method was in the range of 2-12%. The calibration curves were obtained in the range of 5-500 microg L(-1) with reasonable linearity (R2>0.998) and the limits of detection (LODs) ranged between 0.5 and 0.7 microg L(-1) (based on S/N=3). Finally, the applicability of the proposed method was evaluated by extraction and determination of the drugs in urine, plasma and tap water samples. The results indicated that hollow fiber microextraction method has excellent clean-up and high-preconcentration factor and can be served as a simple and sensitive method for monitoring of antidepressant drugs in the biological samples.
评价了基于中空纤维的液相微萃取（HF-LPME）用于在通过HPLC-UV测定三种抗抑郁药物（阿米替林、丙咪嗪和舍曲林）之前的萃取和预富集的适用性。将目标药物从11.0 mL pH为12.0的水溶液（源相）中萃取到浸渍在中空纤维孔中的有机萃取溶剂（正十二烷）中，最后反萃取到位于中空纤维内腔的24 μL水溶液中，并用0.1M的H3PO4将其pH调至2.1（接收相）。萃取是由于中空纤维膜内外的pH梯度而进行的。为了获得高萃取效率，研究并优化了影响HF-LPME的参数，包括源相和接收相的pH、有机相类型、离子强度和源相体积、搅拌速率和萃取时间。在优化条件下，富集因子高达300，该方法的相对标准偏差（R.S.D.%）在2%-12%范围内。校准曲线在5-500 μg L-1范围内获得，具有合理的线性（R2>0.998），检测限（LODs）在0.5至0.7 μg L-1之间（基于S/N=3）。最后，通过萃取和测定尿液、血浆和自来水样品中的药物来评估所提出方法的适用性。结果表明，中空纤维微萃取方法具有出色的净化效果和高预富集因子，可作为一种简单灵敏的方法用于监测生物样品中的抗抑郁药物。
