Pan Gang, Lou Chengfu
College of Animal Sciences, Zhejiang University, Hangzhou, PR China.
J Plant Physiol. 2008 Jul 31;165(11):1204-13. doi: 10.1016/j.jplph.2007.02.012. Epub 2007 Nov 7.
Mulberry (Morus alba) is an important crop tree involved in sericulture and pharmaceuticals. To further understand the development and the environmental adaptability mechanism of mulberry, a cDNA of the gene MaACO1 encoding 1-aminocyclopropane-1-carboxylate oxidase was isolated from mulberry. This was used to investigate stress-responsive expression in mulberry. Developmental expression of ACC oxidase in mulberry leaves and spatial expression in mulberry flowers were also investigated. Damage and low-temperature treatment promoted the expression of MaACO1 in mulberry. In leaves, expression of the MaACO1 gene increased in cotyledons and the lowest leaves with leaf development, but showed reduced levels in emerging leaves. In flowers, the pollinated stigma showed the highest expression level, followed by the unpollinated stigma, ovary, and immature flowers. These results suggest that high MaACO1 expression may be predominantly associated with tissue aging or senescence in mulberry.
桑树（Morus alba）是一种与养蚕业和制药业相关的重要经济作物。为了进一步了解桑树的发育和环境适应机制，从桑树中分离出了编码1-氨基环丙烷-1-羧酸氧化酶的基因MaACO1的cDNA。利用该基因研究了桑树中的胁迫响应表达。还研究了ACC氧化酶在桑叶中的发育表达和在桑花中的空间表达。损伤和低温处理促进了桑树中MaACO1的表达。在叶片中，MaACO1基因的表达在子叶和最下部叶片中随着叶片发育而增加，但在新出现的叶片中表达水平降低。在花中，授粉柱头的表达水平最高，其次是未授粉柱头、子房和未成熟花。这些结果表明，MaACO1的高表达可能主要与桑树的组织衰老有关。
