Fischer Beat B, Krieger-Liszkay Anja, Hideg Eva, Snyrychová Iva, Wiesendanger Manuela, Eggen Rik I L
Department of Environmental Toxicology, Eawag, Swiss Federal Institute of Aquatic Science and Technology, Ueberlandstrasse 133, 8600, Dübendorf, Switzerland.
FEBS Lett. 2007 Dec 11;581(29):5555-60. doi: 10.1016/j.febslet.2007.11.003. Epub 2007 Nov 13.
High light illumination of photosynthetic organisms stimulates the production of singlet oxygen by photosystem II and causes photooxidative stress. In Chlamydomonas reinhardtii, singlet oxygen also induces the expression of the nuclear-encoded glutathione peroxidase homologous gene GPXH. We provide evidence that singlet oxygen stimulates GPXH expression by activating a signaling mechanism outside the thylakoid membrane. Singlet oxygen from photosystem II could be detected with specific probes in the aqueous phase of isolated thylakoid suspensions and the cytoplasm of high light stressed cells. This indicates that singlet oxygen can stimulate a response farther from its production site than generally believed.
光合生物的高光照射会刺激光系统II产生单线态氧，并导致光氧化应激。在莱茵衣藻中，单线态氧还会诱导核编码的谷胱甘肽过氧化物酶同源基因GPXH的表达。我们提供的证据表明，单线态氧通过激活类囊体膜外的信号传导机制来刺激GPXH的表达。用特异性探针可在分离的类囊体悬浮液的水相和高光胁迫细胞的细胞质中检测到来自光系统II的单线态氧。这表明单线态氧能够在比通常认为的更远的距离刺激反应。
