Prestin对膜力学和机电学的影响。

Effects of prestin on membrane mechanics and electromechanics.

作者信息

Anvari Bahman, Zhang Rui, Qian Feng, Rajagopalan Lavanya, Pereira Fred A, Brownell William E

机构信息

Department of Bioengineering, University of California, Riverside, CA 92521, USA.

出版信息

Annu Int Conf IEEE Eng Med Biol Soc. 2007;2007:5384-6. doi: 10.1109/IEMBS.2007.4353559.

DOI:10.1109/IEMBS.2007.4353559

PMID:18003225

Abstract

The electromotility of cochlear outer hair cells (OHCs) is a required process for normal hearing, and involves a membrane-based mechanism in which the transmembrane protein, prestin, plays a central role. We have investigated the contribution of prestin to the mechanics and electromechanics of the cell membrane using membrane tethers formed from human embryonic kidney cells. Our results suggest that prestin appears to change membrane tension and amplify electrically-evoked force generation, while a single point mutation of alanine to tryptophan in prestin reduces electrically-evoked force generation without affecting the membrane tension. We propose that prestin and membrane work in synergy to produce the electrical and mechanical changes that are required during OHC electromotility.

摘要

耳蜗外毛细胞（OHCs）的电运动是正常听力所必需的过程，涉及一种基于膜的机制，其中跨膜蛋白prestin起着核心作用。我们使用源自人胚胎肾细胞形成的膜栓来研究prestin对细胞膜力学和机电学的贡献。我们的结果表明，prestin似乎会改变膜张力并放大电诱发的力产生，而prestin中丙氨酸到色氨酸的单点突变会减少电诱发的力产生，而不影响膜张力。我们提出，prestin与膜协同作用，以产生OHC电运动过程中所需的电学和力学变化。