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颞叶神经递质调节可恢复长期培养的成年脊髓神经元的功能活性。

Temporal neurotransmitter conditioning restores the functional activity of adult spinal cord neurons in long-term culture.

作者信息

Das Mainak, Bhargava Neelima, Bhalkikar Abhijeet, Kang Jung Fong, Hickman James J

机构信息

NanoScience Technology Center, University of Central Florida, Orlando, FL 32826, USA.

出版信息

Exp Neurol. 2008 Jan;209(1):171-80. doi: 10.1016/j.expneurol.2007.09.019. Epub 2007 Sep 26.

Abstract

The ability to culture functional adult mammalian spinal cord neurons represents an important step in the understanding and treatment of a spectrum of neurological disorders including spinal cord injury. Previously, the limited functional recovery of these cells, as characterized by a diminished ability to initiate action potentials and to exhibit repetitive firing patterns, has arisen as a major impediment to their physiological relevance. In this report, we demonstrate that single temporal doses of the neurotransmitters serotonin, glutamate (N-acetyl-DL-glutamic acid) and acetylcholine-chloride lead to the full electrophysiological functional recovery of adult mammalian spinal cord neurons, when they are cultured under defined serum-free conditions. Approximately 60% of the neurons treated regained their electrophysiological signature, often firing single, double and, most importantly, multiple action potentials.

摘要

培养功能性成年哺乳动物脊髓神经元的能力是理解和治疗包括脊髓损伤在内的一系列神经系统疾病的重要一步。此前,这些细胞有限的功能恢复,表现为引发动作电位和展现重复放电模式的能力减弱,已成为其生理相关性的主要障碍。在本报告中,我们证明,当成年哺乳动物脊髓神经元在特定无血清条件下培养时,单剂量的神经递质血清素、谷氨酸(N-乙酰-DL-谷氨酸)和乙酰胆碱氯可使它们实现完全的电生理功能恢复。约60%接受处理的神经元恢复了其电生理特征,常常能产生单个、双个,最重要的是多个动作电位。

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