Katiyar Shashwat, Awasthi S K, Sahu Ranjeev K
Department of Biochemistry, Institute of Bioscience and Biotechnology, C S J M University, Kanpur, U.P., 208024 India.
Sci Total Environ. 2008 Feb 15;390(2-3):355-61. doi: 10.1016/j.scitotenv.2007.10.031. Epub 2007 Nov 26.
The toxic metals alter the immune response of animals as well as humans. In addition to the well documented and numerous toxic effect of chromium on various target organs, number of studies shown that acute and chronic exposure to inorganic chromium may result in impairment of immune functions in the experimental systems. Immunosuppression appears to be more subtle effect of exposure to heavy metals. Therefore, we have taken two different groups of chromium exposed individuals. These were leather tanning workers and chromeplaters. These groups of individuals were regularly exposed to chromium. All the leather tanning workers were highly exposed to chromium having a mean of 96.60+/-113.95 mg/dl (range 12.4-417.21 mg/dl). A suspension of exposed and unexposed human PBMC (0.5x10(6) cells/ml) prepared and cultured in RPMI-1640 supplemented with 10% FCS for 18 h in the presence or absence of PHA (5 microg/ml) and LPS (10 ng/ml) which used for stimulation of IL-2, IL-4, TNF-alpha, IL-10 and IL-6, respectively. The levels of Th1/Th2 cytokine: IL-2, IL-4, TNF-alpha, IL-10 and IL-6 were evaluated in the sera and PHA/LPS stimulated culture supernatant of human PBMCs of chromium exposed workers. In these workers the level of IL-6 was 543.95+/-123.75 pg/ml and 388.40+/-61.24 pg/ml in PHA/LPS stimulated culture supernatant of normal individuals and highly chromium exposed workers, which was significant (P<0.05). This observation suggests that IL-6 levels were suppressed in chromium exposed groups as compared to unexposed healthy volunteers. Although the level of IL-2 in PHA stimulated culture supernatant of PBMCs was suppressed in chromium exposed individuals but it was not significant, IL-4 and IL-10 could not be detected. However, there was no difference in TNF-alpha levels in sera samples as well as unstimulated culture supernatant of h-PBMCs of chromium exposed individuals as compared to control.
有毒金属会改变动物和人类的免疫反应。除了铬对各种靶器官具有充分记录且众多的毒性作用外，大量研究表明，在实验系统中，急性和慢性接触无机铬可能导致免疫功能受损。免疫抑制似乎是接触重金属更为微妙的影响。因此，我们选取了两组不同的铬暴露个体。他们是皮革鞣制工人和镀铬工人。这些个体经常接触铬。所有皮革鞣制工人都高度暴露于铬，平均水平为96.60±113.95毫克/分升（范围为12.4 - 417.21毫克/分升）。制备暴露和未暴露的人外周血单核细胞（PBMC）悬浮液（0.5×10⁶细胞/毫升），并在补充有10%胎牛血清的RPMI - 1640培养基中培养18小时，分别在存在或不存在用于刺激IL - 2、IL - 4、TNF -α、IL - 10和IL - 6的PHA（5微克/毫升）和LPS（10纳克/毫升）的情况下进行培养。在铬暴露工人的人PBMC的血清以及PHA/LPS刺激的培养上清液中评估Th1/Th2细胞因子IL - 2、IL - 4、TNF -α、IL - 10和IL - 6的水平。在这些工人中，正常个体和高铬暴露工人的PHA/LPS刺激培养上清液中IL - 6水平分别为543.95±123.75皮克/毫升和388.40±61.24皮克/毫升，差异具有统计学意义（P<0.05）。这一观察结果表明，与未暴露的健康志愿者相比，铬暴露组的IL - 6水平受到抑制。虽然铬暴露个体的PBMC在PHA刺激培养上清液中的IL - 2水平受到抑制，但差异不显著，未检测到IL - 4和IL - 10。然而，与对照组相比，铬暴露个体的血清样本以及人PBMC未刺激培养上清液中的TNF -α水平没有差异。
Zotero 插件