Lai Xuxin, Zheng Yiwu, Søndergaard Ib, Josephsen Henrik, Løwenstein Henning, Larsen Jørgen Nedergaard, Ipsen Henrik, Jacobsen Susanne
Enzyme and Protein Chemistry Group, BioCentrum-DTU, Building 224, Søltofts Plads, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
Vaccine. 2007 Dec 17;25(52):8732-40. doi: 10.1016/j.vaccine.2007.10.026. Epub 2007 Nov 5.
A method for determining the aluminium content of an aluminium hydroxide suspension using near infrared (NIR) transmittance spectroscopy has been developed. Inductively coupled plasma-atomic emission spectroscopy (ICP-AES) was used as reference method. The factors influencing the NIR analysis, such as different sample containers (transmission cell and cuvette), sedimentation in the suspension, day-to-day variation and batch-to-batch variation have been studied before constructing a calibration model. Seven dilutions (0-4100 mg Al/L) of five batches of aluminium hydroxide suspension samples were measured by NIR transmission each on five different days, with total of 175 spectra used for the calibration set. The multivariate data analysis technique partial least square regression (PLSR) was applied to build the calibration model. Six batches of aluminium hydroxide samples were used for the test set. ICP-AES and NIR transmittance spectroscopy exhibit comparable precision and accuracy. The NIR method provides several advantages: no complicated sample preparation; easy to operate; fast and non-destructive. In conclusion, NIR transmittance spectroscopy can be an alternative analytical method for determining aluminium content in aluminium hydroxide suspension.
已开发出一种使用近红外（NIR）透射光谱法测定氢氧化铝悬浮液中铝含量的方法。电感耦合等离子体原子发射光谱法（ICP-AES）用作参考方法。在构建校准模型之前，研究了影响近红外分析的因素，如不同的样品容器（透射池和比色皿）、悬浮液中的沉降、每日变化和批次间变化。对五批氢氧化铝悬浮液样品的七种稀释液（0 - 4100 mg Al/L）在五个不同日期分别进行近红外透射测量，总共175个光谱用于校准集。应用多元数据分析技术偏最小二乘回归（PLSR）构建校准模型。六批氢氧化铝样品用于测试集。ICP-AES和近红外透射光谱法具有相当的精密度和准确度。近红外方法具有几个优点：无需复杂的样品制备；易于操作；快速且无损。总之，近红外透射光谱法可作为测定氢氧化铝悬浮液中铝含量的替代分析方法。
