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人DBP与25-羟基维生素D结合时结构域之间的相互作用。

Cross-talk among structural domains of human DBP upon binding 25-hydroxyvitamin D.

作者信息

Ray Arjun, Swamy Narasimha, Ray Rahul

机构信息

Bioorganic Chemistry & Structural Biology, Department of Medicine, Boston University School of Medicine, 85 East Newton Street, Boston, MA 02118, USA.

出版信息

Biochem Biophys Res Commun. 2008 Jan 25;365(4):746-50. doi: 10.1016/j.bbrc.2007.11.033. Epub 2007 Nov 21.

DOI:10.1016/j.bbrc.2007.11.033
PMID:18035050
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2174211/
Abstract

Serum vitamin D-binding protein (DBP) is structurally very similar to serum albumin (ALB); both have three distinct structural domains and high cysteine-content. Yet, functionally they are very different. DBP possesses high affinity for vitamin D metabolites and G-actin, but ALB does not. It has been suggested that there may be cross-talk among the domains so that binding of one ligand may influence the binding of others. In this study we have employed 2-p-toluidinyl-6-sulfonate (TNS), a reporter molecule that fluoresces upon binding to hydrophobic pockets of DBP. We observed that recombinant domain III possesses strong binding for TNS, which is not influenced by 25-hydroxyvitamin D(3) (25-OH-D(3)), yet TNS fluorescence of the whole protein is quenched by 25-OH-D(3). These results provide a direct evidence of cross-talk among the structural domains of DBP.

摘要

血清维生素D结合蛋白（DBP）在结构上与血清白蛋白（ALB）非常相似；两者都有三个不同的结构域且半胱氨酸含量高。然而，它们在功能上却有很大差异。DBP对维生素D代谢物和G-肌动蛋白具有高亲和力，但ALB则不然。有人提出，这些结构域之间可能存在相互作用，使得一种配体的结合可能会影响其他配体的结合。在本研究中，我们使用了2-对甲苯胺基-6-磺酸盐（TNS），一种与DBP疏水口袋结合时会发出荧光的报告分子。我们观察到重组结构域III对TNS具有很强的结合力，这不受25-羟基维生素D3（25-OH-D3）的影响，但整个蛋白的TNS荧光会被25-OH-D3淬灭。这些结果为DBP结构域之间的相互作用提供了直接证据。

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本文引用的文献

1
Selective inhibition of the C5a chemotactic cofactor function of the vitamin D binding protein by 1,25(OH)2 vitamin D3.
Mol Immunol. 2006 Mar;43(8):1109-15. doi: 10.1016/j.molimm.2005.07.023. Epub 2005 Aug 22.
2
Identification of a region in the vitamin D-binding protein that mediates its C5a chemotactic cofactor function.
J Biol Chem. 2004 Dec 17;279(51):53282-7. doi: 10.1074/jbc.M411462200. Epub 2004 Oct 14.
3
Vitamin D binding protein-macrophage activating factor (DBP-maf) inhibits angiogenesis and tumor growth in mice.
Neoplasia. 2003 Jan-Feb;5(1):32-40. doi: 10.1016/s1476-5586(03)80015-5.
4
Actin-DBP: the perfect structural fit?
Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):263-73. doi: 10.1107/s0907444902021455. Epub 2003 Jan 23.
5
Crystal structure of the complex between actin and human vitamin D-binding protein at 2.5 A resolution.
Biochemistry. 2002 Jul 23;41(29):9015-20. doi: 10.1021/bi026054y.
6
Biochemical and preliminary crystallographic characterization of the vitamin D sterol- and actin-binding by human vitamin D-binding protein.
Arch Biochem Biophys. 2002 Jun 1;402(1):14-23. doi: 10.1016/S0003-9861(02)00033-4.
7
Baculovirus-expressed vitamin D-binding protein-macrophage activating factor (DBP-maf) activates osteoclasts and binding of 25-hydroxyvitamin D(3) does not influence this activity.
J Cell Biochem. 2001;81(3):535-46. doi: 10.1002/1097-4644(20010601)81:3<535::aid-jcb1067>3.0.co;2-6.
8
Antitumor effect of vitamin D-binding protein-derived macrophage activating factor on Ehrlich ascites tumor-bearing mice.
Proc Soc Exp Biol Med. 1999 Jan;220(1):20-6. doi: 10.1046/j.1525-1373.1999.d01-3.x.
9
Bacterial expression of human vitamin D-binding protein (Gc2) in functional form.
Protein Expr Purif. 1997 Jun;10(1):115-22. doi: 10.1006/prep.1996.0720.
10
Molecular recognition in vitamin D-binding protein.
Proc Soc Exp Biol Med. 1996 Sep;212(4):305-12. doi: 10.3181/00379727-212-44020.

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