Yu Fei, Liao Yingjun, Jin Yaping, Zhao Yue, Ren Yahao, Lu Chunwei, Li Gexin, Li Yanxi, Yang Jun
Department of Nutrition and Food Hygiene, School of Public Health, China Medical University, No. 92 Beier Road, Heping District, Shenyang, Liaoning Postal code: 110001, People's Republic of China.
Arch Toxicol. 2008 Jul;82(7):453-9. doi: 10.1007/s00204-007-0267-5. Epub 2007 Nov 29.
To examine the effects of meso-2,3-dimercaptosuccinic acid (DMSA) on developmental toxicity resulting from exposure to lead in utero, female albino mice were exposed to lead by drinking water contaminated with lead acetate for 4 weeks. After the cessation of lead exposure, female mice were supplemented by gavage with saline solution, DMSA, or DMSA and calcium as well as ascorbic acid from the fourth day of gestation until parturition, respectively. Lead levels (blood, liver, and bone) were measured at birth. Pups were then tested about neural development including surface righting reflex, cliff avoidance and air righting reflex. The markers of physical maturation, such as body weight, pinna unfolding, incisor eruption, and eye opening were also recorded. DMSA treatment decreased blood lead levels of pregnant mice, however, increased lead levels in both liver and bone of fetus, and delayed the early physical and neural development of offspring. Calcium and ascorbic acid reduced the transfer of lead to fetus. In conclusion, DMSA treatment during pregnancy enhances lead-induced fetal developmental toxicity.
为研究中-2,3-二巯基丁二酸（DMSA）对子宫内铅暴露所致发育毒性的影响，将雌性白化小鼠通过饮用含醋酸铅的污染水暴露于铅环境中4周。停止铅暴露后，从妊娠第4天直至分娩，分别通过灌胃给雌性小鼠补充生理盐水、DMSA、DMSA与钙以及抗坏血酸。在出生时测量铅水平（血液、肝脏和骨骼）。然后对幼崽进行神经发育测试，包括表面翻正反射、悬崖回避和空中翻正反射。还记录了身体成熟的指标，如体重、耳廓展开、门齿萌出和睁眼情况。DMSA治疗降低了怀孕小鼠的血铅水平，但增加了胎儿肝脏和骨骼中的铅水平，并延迟了后代的早期身体和神经发育。钙和抗坏血酸减少了铅向胎儿的转移。总之，孕期进行DMSA治疗会增强铅诱导的胎儿发育毒性。
