类嗜异性抗体对类胰蛋白酶免疫测定的干扰

Heterophilic antibody interference in a tryptase immunoassay.

作者信息

van Toorenenbergen Albert W, Hooijkaas Herbert, Heerenbrink Gerard Klein, Dufour-van den Goorbergh Diana M

机构信息

Department of Clinical Chemistry, Erasmus University Medical Center, Rotterdam, The Netherlands.

出版信息

Clin Biochem. 2008 Mar;41(4-5):331-4. doi: 10.1016/j.clinbiochem.2007.11.003. Epub 2007 Nov 17.

DOI:10.1016/j.clinbiochem.2007.11.003

PMID:18054333

Abstract

OBJECTIVE

Investigation of the susceptibility of a tryptase immunoassay to interference by heterophilic antibodies.

METHODS

The effect of preincubation with a blocking agent was investigated on the levels of tryptase, human anti-mouse antibodies and IgM rheumatoid factor in sera with elevated IgM rheumatoid factor levels.

RESULTS

In 5 of 30 sera with IgM rheumatoid factor, tryptase levels were reduced at least twofold after pre-incubation with blocking reagent. A significant association was observed between the presence of IgM rheumatoid factor in the sera and the interference of tryptase immunoassay. There was no quantitative correlation found between the reduction in serum tryptase level by treatment with a blocking agent, and the amount of IgM rheumatoid factor was present. However, this reduction in serum tryptase was significantly correlated with the amount of human anti-mouse antibodies in the sera. After incubation with blocking agent, there was no change in IgM Rheumatoid factor level, but a significant decrease in human anti-mouse antibodies.

CONCLUSION

The Phadia tryptase assay method, in its present form, is sensitive to interference by heterophilic antibodies.

摘要

目的

研究类胰蛋白酶免疫测定法对嗜异性抗体干扰的敏感性。

方法

研究了用封闭剂预孵育对类风湿因子IgM水平升高的血清中类胰蛋白酶、人抗小鼠抗体和IgM类风湿因子水平的影响。

结果

在30份类风湿因子IgM血清中，有5份在与封闭试剂预孵育后类胰蛋白酶水平至少降低了两倍。血清中类风湿因子IgM的存在与类胰蛋白酶免疫测定的干扰之间存在显著关联。用封闭剂处理后血清类胰蛋白酶水平的降低与类风湿因子IgM的含量之间未发现定量相关性。然而，血清类胰蛋白酶的这种降低与血清中人抗小鼠抗体的量显著相关。与封闭剂孵育后，IgM类风湿因子水平无变化，但人抗小鼠抗体显著减少。