Conde Pamela A, Herrera Carolina, Trasorras Virginia L, Giuliano Susana M, Director Ariel, Miragaya Marcelo H, Chaves María G, Sarchi María I, Stivale Diana, Quintans Carlos, Agüero Alicia, Rutter Bruno, Pasqualini Sergio
Halitus Biotecnología, Halitus Instituto Medico, Capital Federal, Argentina.
Anim Reprod Sci. 2008 Dec;109(1-4):298-308. doi: 10.1016/j.anireprosci.2007.10.004. Epub 2007 Oct 23.
The interest for South American camelids has increased in the last years. The aim of the present research was to compare the in vitro production of Lama glama embryos using two techniques: in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). For IVF technique, we compared the effect of adding or not, heparin, penicillamine and hypotaurine as sperm capacitating agents. In the oocyte group subjected to ICSI, activation with or without, ionomycin and 6-dimethylaminopurine (6-DMAP) was assessed. Semen samples were obtained by electroejaculation and incubated at 38 degrees C in a 25% (v/v) collagenase solution. The cleavage and embryo development rates were compared between the different experimental groups. Only the number of cleaved oocytes was less when ICSI with no activation was used (p<0.05).
在过去几年里，人们对南美洲骆驼科动物的兴趣有所增加。本研究的目的是使用两种技术比较原驼胚胎的体外生产：体外受精（IVF）和胞浆内精子注射（ICSI）。对于IVF技术，我们比较了添加或不添加肝素、青霉胺和亚牛磺酸作为精子获能剂的效果。在接受ICSI的卵母细胞组中，评估了使用或不使用离子霉素和6-二甲基氨基嘌呤（6-DMAP）进行激活的情况。通过电刺激采精获取精液样本，并在38℃下于25%（v/v）胶原酶溶液中孵育。比较了不同实验组之间的卵裂率和胚胎发育率。仅在未进行激活的ICSI组中，卵裂的卵母细胞数量较少（p<0.05）。
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