用于将腺嘌呤核苷的甲酰乙烯基和羧基乙烯基加合物掺入寡核苷酸的修饰核苷的合成。

Synthesis of modified nucleosides for incorporation of formyletheno and carboxyetheno adducts of adenine nucleosides into oligonucleotides.

作者信息

Koissi Niangoran, Lönnberg Harri

机构信息

Department of Chemistry, University of Turku, Turku, Finland.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2007;26(8-9):1203-6. doi: 10.1080/15257770701527836.

DOI:10.1080/15257770701527836

PMID:18058566

Abstract

Three protected derivatives of 1,N(6)-ethenoadenine nucleosides, viz. 3-[5-O-(4,4'-dimethoxytrityl) of 7-formyl-(1) and 7-(1,2-diacetyloxypropyl)-2'-deoxyadenosine (2), and 3-[5-O-(4,4'-dimethoxytrityl)-2-O-(tert-butyldimethylsilyl)-7-(ethoxycarbonyl)adenosine (3), expected to allow introduction of formyletheno and carboxyethenoadenine adducts into oligonucleotides by the conventional phosphoramidite chemistry, have been synthesized.

摘要

1,N(6)-乙烯腺嘌呤核苷的三种受保护衍生物，即7-甲酰基-3-[5-O-(4,4'-二甲氧基三苯甲基)]-2'-脱氧腺苷(1)、7-(1,2-二乙酰氧基丙基)-2'-脱氧腺苷(2)，以及3-[5-O-(4,4'-二甲氧基三苯甲基)-2-O-(叔丁基二甲基甲硅烷基)-7-(乙氧羰基)腺苷(3)，有望通过传统亚磷酰胺化学方法将甲酰乙烯基和羧基乙烯基腺嘌呤加合物引入寡核苷酸中，现已合成。