Kim Jong-Eun, Hur Haeng Jeon, Lee Ki Won, Lee Hyong Joo
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea.
J Microbiol Biotechnol. 2007 Sep;17(9):1491-7.
Arginine deiminase (ADI, E.C. 3.5.3.6), one of the arginine deprivation enzymes, exhibits anticarcinogenic activities. The present study investigated the anti-inflammatory activities of the purified recombinant ADI originating from Lactococcus lactis ssp. lactis ATCC7962 (LADI). LADI dose-dependently inhibited lipopolysaccharide (LPS)-induced upregulation of inducible nitric oxide synthase and the production of nitric oxide in RAW 264.7 murine macrophages. The induction of cyclooxygenase-2 expression and subsequent production of prostaglandin E2 by LPS was also attenuated by LADI treatment. Moreover, LADI inhibited the production of interleukin-6 in LPS-stimulated RAW 264.7 macrophages. These results indicate that LADI exerts anti-inflammatory effects, which may in part explain its chemopreventive potential.
精氨酸脱亚氨酶（ADI，E.C. 3.5.3.6）是一种精氨酸剥夺酶，具有抗癌活性。本研究调查了源自乳酸乳球菌乳酸亚种ATCC7962（LADI）的纯化重组ADI的抗炎活性。LADI剂量依赖性地抑制脂多糖（LPS）诱导的RAW 264.7小鼠巨噬细胞中诱导型一氧化氮合酶的上调和一氧化氮的产生。LADI处理还减弱了LPS诱导的环氧化酶-2表达及随后前列腺素E2的产生。此外，LADI抑制了LPS刺激的RAW 264.7巨噬细胞中白细胞介素-6的产生。这些结果表明，LADI具有抗炎作用，这可能部分解释了其化学预防潜力。
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