Yuan Hai-Yan, Huang Su-Zhen, Guo Zhi, Han Yu-Lin
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences & Nanjing Sun Yat-san Botanical Garden, Nanjing 210014, China.
Ying Yong Sheng Tai Xue Bao. 2007 Sep;18(9):2111-6.
A solution culture experiment was conducted to examine the effects of Zn (1-100 mg x L(-1)) on the growth, Cd accumulation, and antioxidant enzyme activities of Iris lactea var. chinensis under Cd stress (10 mg x L(-1)). The results showed that after the addition of Zn, the Cd content changed a little in shoots, but increased significantly in roots, with a trend of rising first and dropping then. When treated with 1 mg Zn x L(-1), the Cd content in roots was the highest, being 51.4% higher than the control. When the Zn concentration was higher than 1 mg x L(-1), the Cd content in roots decreased to some extent, but was still higher than the control. Low concentration (1-10 mg x L(-1)) Zn increased the shoot biomass and leaf chlorophyll content, but decreased the malondialdehyde (MDA) content. At 10 mg Zn x L(-1), the chlorophyll a and b contents went up to the maximum, being 5.21% and 22.27% higher than the control, respectively, while the MDA content decreased by 25.46%, indicating that low concentration Zn could alleviate Cd damage. With increasing Zn concentration, the alleviation effect of Zn on Cd damage decreased gradually. When the Zn concentration was 100 mg x L(-1), I. lactea var. chinensis was damaged most seriously, with the biomass and chlorophyll content reduced and MDA content increased significantly. Within the ranges of test Cd and Zn concentrations, the activities of superoxide dismutase (SOD) and peroxidase (POD) had an increasing trend, suggesting that SOD and POD played an important antioxidant protective role for Cd or Zn-Cd stressed I. lactea var. chinensis.
进行了一项溶液培养实验,以研究锌(1-100毫克/升)对镉胁迫(10毫克/升)下马蔺生长、镉积累及抗氧化酶活性的影响。结果表明,添加锌后,地上部镉含量变化不大,但根部镉含量显著增加,呈先升后降趋势。用1毫克/升锌处理时,根部镉含量最高,比对照高51.4%。当锌浓度高于1毫克/升时,根部镉含量有所下降,但仍高于对照。低浓度(1-10毫克/升)锌增加了地上部生物量和叶片叶绿素含量,但降低了丙二醛(MDA)含量。在10毫克/升锌处理时,叶绿素a和b含量分别升至最高,比对照分别高5.21%和22.27%,而MDA含量下降了25.46%,表明低浓度锌可缓解镉毒害。随着锌浓度增加,锌对镉毒害的缓解作用逐渐减弱。当锌浓度为100毫克/升时,马蔺受伤害最严重,生物量和叶绿素含量降低,MDA含量显著增加。在试验的镉和锌浓度范围内,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性呈上升趋势,表明SOD和POD对镉或锌-镉胁迫下的马蔺发挥了重要的抗氧化保护作用。