菲律宾野生斑节对虾中白斑综合征病毒（WSSV）的流行情况。

Prevalence of white spot syndrome virus (WSSV) in wild shrimp Penaeus monodon in the Philippines.

作者信息

de la Peña Leobert D, Lavilla-Pitogo Celia R, Villar Corina Belle R, Paner Milagros G, Sombito Christopher D, Capulos Geimbo C

机构信息

Southeast Asian Fisheries Development Center, Aquaculture Department, Tigbauan 5021, Iloilo, Philippines.

出版信息

Dis Aquat Organ. 2007 Oct 15;77(3):175-9. doi: 10.3354/dao01834.

DOI:10.3354/dao01834

PMID:18062468

Abstract

Prevalence of white spot syndrome virus (WSSV) was determined using polymerase chain reaction (PCR) methodology on DNA extracted from the gills of wild black tiger shrimp Penaeus monodon collected from 7 sampling sites in the Philippines. These 7 sampling sites are the primary sources of spawners and broodstock for hatchery use. During the dry season, WSSV was detected in shrimp from all sites except Bohol, but during the wet season it was not detected in any site except Palawan. None of the WSSV-PCR positive shrimp showed signs of white spots in the cuticle. Prevalence of WSSV showed seasonal variations, i.e. prevalence in dry season (April to May) was higher than in the wet season (August to October). These results suggest that WSSV has already become established in the local marine environment and in wild populations of P. monodon. Thus, broodstock collected during the dry season could serve as the main source of WSSV contamination in shrimp farms due to vertical transmission of the virus in hatcheries.

摘要

采用聚合酶链反应（PCR）方法，对从菲律宾7个采样点采集的野生黑虎虾（斑节对虾）鳃中提取的DNA进行检测，以确定白斑综合征病毒（WSSV）的流行情况。这7个采样点是孵化场用作亲虾和种虾的主要来源。在旱季，除薄荷岛外，所有采样点的虾均检测到WSSV，但在雨季，除巴拉望省外，其他任何地点均未检测到。WSSV-PCR阳性的虾在表皮均未出现白斑迹象。WSSV的流行呈现季节性变化，即旱季（4月至5月）的流行率高于雨季（8月至10月）。这些结果表明，WSSV已在当地海洋环境和斑节对虾野生种群中定殖。因此，由于该病毒在孵化场中的垂直传播，旱季采集的亲虾可能成为对虾养殖场WSSV污染的主要来源。