Ren Xiu-lian, Xu Yan-wen, Zhou Can-quan, Zhuang Guang-lun
Reproductive Medical Center, the First Affiliated Hospital, SUN Yat-sen University, Guangzhou, Guangdong, 510080 PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2007 Dec;24(6):706-8.
To investigate the mechanism and factors affecting mosaicism in human preimplantation embryos by using 2 sequential rounds of fluorescence in situ hybridization(FISH).
Totally 51 normal fertilized embryos, which were not suitable for embryo transfer and cryopreservation, were analyzed on day 3 after fertilization by using two sequential rounds of FISH. Chromosomes 13, 16, 18, 21, 22, X and Y were analyzed.
Among 51 embryos, 16 (31.4%) were mosaic, 12 (23.5%) were chaotic, and the remaining were either normal (27.5%) or non-mosaic abnormal (17.6%). The incidence of mosaic embryos was related to embryo developmental stage, for the incidence of mosaicism increased from 12.5% in embryos <or= 4 cell stage to 40.0% in 5-8 cell stage embryos. The aneuploidy rate for the patients over 35 years of age was significantly higher than that of the patients under 35 years (57.1% vs 23.3%).
Mosaicism is common in human preimplantaion embryos, which may be one of the important factors affecting the success rates in IVF-ET. Most of the chromosomal abnormalities can be identified by two sequential rounds of FISH.
通过两轮连续的荧光原位杂交（FISH）研究人类植入前胚胎中影响嵌合体形成的机制和因素。
选取51个不适合胚胎移植和冷冻保存的正常受精胚胎，在受精后第3天利用两轮连续的FISH进行分析。分析的染色体包括13、16、18、21、22、X和Y染色体。
51个胚胎中，16个（31.4%）为嵌合体，12个（23.5%）为混乱型，其余为正常（27.5%）或非嵌合异常（17.6%）。嵌合胚胎的发生率与胚胎发育阶段有关，因为嵌合体发生率从≤4细胞期胚胎的12.5%增加到5 - 8细胞期胚胎的40.0%。35岁以上患者的非整倍体率显著高于35岁以下患者（57.1%对23.3%）。
嵌合体在人类植入前胚胎中很常见，这可能是影响体外受精 - 胚胎移植成功率的重要因素之一。两轮连续的FISH可识别大多数染色体异常情况。
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