Soler A J, Poulin N, Fernández-Santos M R, Cognie Y, Esteso M C, Garde J J, Mermillod P
Grupo de Biología de la Reproducción, Instituto de Investigación en Recursos Cinegéticos, IREC, (CSIC-UCLM-JCCM), Campus Universitario, Albacete, SpainUnité de Physiologie de la Reproduction et des Comportements, INRA, Nouzilly, France.
Reprod Domest Anim. 2008 Jun;43(3):293-298. doi: 10.1111/j.1439-0531.2007.00893.x. Epub 2007 Dec 7.
A heterologous in vitro system, using zona-intact sheep oocytes, was used to evaluate the relationship between sperm factors of Iberian red deer thawed epididymal sperm and the percentage of cleaved oocytes. Epididymal spermatozoa were recovered from six males, diluted with freezing extender and cryopreserved. After thawing sperm motility (SM) and acrosome and membrane integrities were evaluated. Again, these parameters were assessed after incubation in freezing extender at 37 degrees C for 2 h. After cryopreservation the values for SM and acrosome and membrane integrities were high (approximately 80, 80 and 70% respectively). However, these values significantly decreased after incubation (approximately 59, 62 and 47% respectively). Red deer thawed epididymal sperm fertilized zona-intact sheep oocytes, although the percentage of cleaved oocytes was low (approximately 22%). No relationship was found between sperm parameters assessed after thawing and the percentage of cleaved oocytes. Likewise, any sperm parameter evaluated after incubation was assessed in relation to the percentage of cleaved oocytes. However, acrosome and membrane integrities were near to significance (p = 0.06 and p = 0.09 respectively). Then, we conducted a reduced model with these two variables and both were related to the percentage of cleaved oocytes (p = 0.02 and p = 0.04 respectively). Thus, acrosome and membrane integrities were related to the percentage of cleaved oocytes negatively and positively respectively. It was concluded that the classical parameters assessed in deer thawed sperm samples can be good predictors of the ability to fertilize zona-intact sheep oocytes.
使用一种异源体外系统，即完整透明带的绵羊卵母细胞，来评估伊比利亚马鹿解冻附睾精子的精子因素与卵裂卵母细胞百分比之间的关系。从六只雄性马鹿中采集附睾精子，用冷冻稀释液稀释后进行冷冻保存。解冻后评估精子活力（SM）、顶体和膜完整性。同样，在37℃的冷冻稀释液中孵育2小时后再次评估这些参数。冷冻保存后，SM、顶体和膜完整性的值较高（分别约为80%、80%和70%）。然而，孵育后这些值显著下降（分别约为59%、62%和47%）。马鹿解冻附睾精子使完整透明带的绵羊卵母细胞受精，尽管卵裂卵母细胞的百分比很低（约22%）。解冻后评估的精子参数与卵裂卵母细胞的百分比之间未发现相关性。同样，孵育后评估的任何精子参数都与卵裂卵母细胞的百分比相关。然而，顶体和膜完整性接近显著水平（分别为p = 0.06和p = 0.09）。然后，我们用这两个变量构建了一个简化模型，两者都与卵裂卵母细胞的百分比相关（分别为p = 0.02和p = 0.04）。因此，顶体和膜完整性分别与卵裂卵母细胞的百分比呈负相关和正相关。得出的结论是，在鹿解冻精子样本中评估的经典参数可以很好地预测使完整透明带绵羊卵母细胞受精的能力。
