Amorim Mónica J B, Novais Sara, Römbke Jörg, Soares Amadeu M V M
CESAM-Center for Environmental and Marine Studies, and Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.
Environ Pollut. 2008 Sep;155(1):112-6. doi: 10.1016/j.envpol.2007.10.028. Epub 2007 Dec 11.
Enchytraeids are ecologically relevant soil species and are commonly used in standardized toxicity tests. Their rapid reaction to a chemical exposure can be used as a toxicological measurement endpoint that assesses the avoidance behavior. The objectives of this investigation were to determine the effects of soil properties on the avoidance behavior of Enchytraeus albidus and to optimize the duration of avoidance test. The avoidance tests included (1) exposures in OECD artificial soil with standard or modified properties (pH, clay or peat content), and (2) exposures to copper chloride, cadmium chloride, and to the organic pesticides dimethoate and phenmedipham for different time periods. Results showed that alteration of OECD soil constituents significantly affected the avoidance behavior of enchytraeids, and that the 48-h exposure was the optimal duration of the test. Consideration of soil properties is important for selecting appropriate experimental design and interpreting the results of the enchytraeid avoidance test.
线蚓是具有生态相关性的土壤物种，常用于标准化毒性测试。它们对化学暴露的快速反应可作为评估回避行为的毒理学测量终点。本研究的目的是确定土壤性质对线蚓（Enchytraeus albidus）回避行为的影响，并优化回避试验的持续时间。回避试验包括：（1）在具有标准或改良性质（pH值、粘土或泥炭含量）的经合组织人工土壤中进行暴露，以及（2）在不同时间段内暴露于氯化铜、氯化镉、有机农药乐果和甜菜安。结果表明，经合组织土壤成分的改变显著影响了线蚓的回避行为，48小时暴露是试验的最佳持续时间。考虑土壤性质对于选择合适的实验设计和解释线蚓回避试验结果很重要。
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