[血清抗中性粒细胞胞浆抗体（ANCA）、抗酿酒酵母抗体（ASCA）、抗细菌鞭毛蛋白抗体（ABBA）在特发性肠道炎症诊断中的意义]
[Significance of serum antibodies ANCA, ASCA, ABBA in diagnostics of idiopathic intestinal inflammations].
作者信息
Pintér M, Pintérová Kolesárová M, Drahosová M, Rejchrt S, Douda T, Tachecí I, Kopácová M, Bures J
机构信息
II. Interní klinika LF UK a FN a Subkatedra gastroenterologie, Hradec Králové.
出版信息
Cas Lek Cesk. 2007;146(11):863-7.
BACKGROUND
Crohn's disease and ulcerative colitis still remain a heterogeneous group of diseases with an unclear aetiology. Serologic methods play important role in their diagnosing though there is still not an ideal marker. We tried to determine the importance of serological testing of ASCA IgA, IgG, ANCA, ABBA antibodies in patients with ulcerative colitis and Crohn's disease.
METHODS AND RESULTS
ASCA IgG, ASCA IgA; ANCA, ABBA antibodies and C-reactive protein were detected by indirect fluorescence assay. ASCA IgA, ASCA IgG, ABBA, ANCA were examined in 40 patients (28 Crohn's disease, 12 ulcerative colitis, 32 health controls). Specificity of ASCA IgA, IgG in CD patients was high (both 96.2%), specificity ANCA in UC 100%. ABBA antibodies had low sensitivity and specificity in both diseases. Combination of ASCA, ANCA, ABBA makes the specificity higher.
CONCLUSIONS
We showed the importance of combination ASCA, ANCA with ABBA antibodies to improve the serological diagnosing of IBD.
背景
克罗恩病和溃疡性结肠炎仍是病因不明的一组异质性疾病。血清学方法在其诊断中发挥着重要作用，尽管目前仍没有理想的标志物。我们试图确定抗酿酒酵母抗体IgA、IgG、抗中性粒细胞胞浆抗体、抗β2糖蛋白I抗体（ABBA）检测在溃疡性结肠炎和克罗恩病患者血清学检测中的重要性。
方法与结果
采用间接荧光法检测抗酿酒酵母抗体IgG、抗酿酒酵母抗体IgA、抗中性粒细胞胞浆抗体、抗β2糖蛋白I抗体及C反应蛋白。对40例患者（28例克罗恩病、12例溃疡性结肠炎、32例健康对照）检测抗酿酒酵母抗体IgA、抗酿酒酵母抗体IgG、抗β2糖蛋白I抗体、抗中性粒细胞胞浆抗体。克罗恩病患者中抗酿酒酵母抗体IgA、IgG的特异性较高（均为96.2%），溃疡性结肠炎患者中抗中性粒细胞胞浆抗体的特异性为100%。抗β2糖蛋白I抗体在两种疾病中的敏感性和特异性均较低。抗酿酒酵母抗体、抗中性粒细胞胞浆抗体、抗β2糖蛋白I抗体联合检测可提高特异性。
结论
我们证明了抗酿酒酵母抗体、抗中性粒细胞胞浆抗体与抗β2糖蛋白I抗体联合检测对提高炎症性肠病血清学诊断的重要性。
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