Farrokhi Naser, Whitelegge Julian P, Brusslan Judy A
National Institute of Genetic Engineering and Biotechnology, Pajoohesh Blvd., Tehran-Karaj Highway, 17th Km., Tehran, Iran.
Plant Biotechnol J. 2008 Feb;6(2):105-34. doi: 10.1111/j.1467-7652.2007.00315.x. Epub 2007 Dec 6.
Extracellular plant peptides perform a large variety of functions, including signalling and defence. Intracellular peptides often have physiological functions or may merely be the products of general proteolysis. Plant peptides have been identified and, in part, functionally characterized through biochemical and genetic studies, which are lengthy and in some cases impractical. Peptidomics is a branch of proteomics that has been developed over the last 5 years, and has been used mainly to study neuropeptides in animals and the degradome of proteases. Peptidomics is a fast, efficient methodology that can detect minute and transient amounts of peptides and identify their post-translational modifications. This review describes known plant peptides and introduces the use of peptidomics for the detection of novel plant peptides.
细胞外植物肽具有多种功能,包括信号传导和防御。细胞内肽通常具有生理功能,或者可能仅仅是一般蛋白质水解的产物。植物肽已通过生化和遗传学研究得以鉴定,并在一定程度上进行了功能表征,这些研究耗时较长,且在某些情况下并不实用。肽组学是蛋白质组学的一个分支,在过去5年中得到了发展,主要用于研究动物中的神经肽和蛋白酶的降解组。肽组学是一种快速、高效的方法,能够检测微量和瞬时存在的肽,并识别其翻译后修饰。本文综述了已知的植物肽,并介绍了肽组学在检测新型植物肽中的应用。