Selvakumar G, Kundu S, Gupta Anand D, Shouche Yogesh S, Gupta Hari S
Vivekananda Institute of Hill Agriculture, Indian Council of Agricultural Research, Almora 263601, Uttarakhand, India.
Curr Microbiol. 2008 Feb;56(2):134-9. doi: 10.1007/s00284-007-9062-z. Epub 2007 Dec 11.
The leguminous vine Kudzu (Pueraria thunbergiana) is an introduction into the N. W. Himalayan region of India. Despite its value as a fodder and cover crop, little is known about the nature of the nodule microflora. In an attempt to study the nodule bacteria, we isolated and characterized three nonrhizobial plant growth promoting bacteria from surface sterilized nodules of Kudzu. Based on the sequencing of the 16 S r RNA gene, the isolates were designated as Bacillus thuringiensis KR-1, Enterobacter asburiae KR-3, and Serratia marcescens KR-4. Crystalline bodies were detected in the isolate KR-1, confirming its identity as B. thuringiensis. Under in vitro conditions, all three isolates were found to produce indole acetic acid. Other plant growth promotion attributes such as P solubilization, hydrogen cyanide production, and ammonia production varied among the isolates. All of the three isolates promoted growth and positively influenced nutrient uptake parameters of wheat seedlings.
豆科藤本植物葛藤（Pueraria thunbergiana）是引入印度喜马拉雅西北部地区的植物。尽管它作为饲料和覆盖作物具有价值，但对其根瘤微生物区系的性质了解甚少。为了研究根瘤细菌，我们从葛藤表面消毒的根瘤中分离并鉴定了三种非根瘤菌的植物促生细菌。基于16S rRNA基因测序，这些分离株被命名为苏云金芽孢杆菌KR-1、阿氏肠杆菌KR-3和粘质沙雷氏菌KR-4。在分离株KR-1中检测到晶体，证实其为苏云金芽孢杆菌。在体外条件下，发现所有三种分离株都能产生吲哚乙酸。其他植物促生特性，如磷的溶解、氰化氢的产生和氨的产生，在分离株之间有所不同。所有这三种分离株都促进了小麦幼苗的生长，并对养分吸收参数产生了积极影响。
