Lang Gillian A, Devera T Scott, Lang Mark L
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Blood. 2008 Feb 15;111(4):2158-62. doi: 10.1182/blood-2007-10-117309. Epub 2007 Dec 12.
Activation of natural killer-like T (NKT) cells with the CD1d ligand alpha-galactosylceramide enhances T-dependent humoral immune responses against coadministered T-dependent Ag. At present, there is little information on the mechanisms involved other than a dependence on CD1d expression by antigen-presenting cells and/or development of the NKT subset. We therefore tested the hypothesis that direct presentation of alpha-GC by B cells was required for NKT-enhanced Ab responses against T-dependent Ag. We reconstituted B cell-deficient microMT mice with B cells from C57Bl/6 donors or CD1d(-/-) donors before immunization with NP-KLH alone or NP-KLH mixed with alpha-GC. We made the surprising observation that B-cell expression of CD1d is absolutely required for the NKT-enhanced Ab response. Our data show that the mechanism by which NKT cells enhance humoral immune responses involves interaction with CD1d-expressing B cells.
用CD1d配体α-半乳糖神经酰胺激活自然杀伤样T（NKT）细胞可增强针对共同给予的T依赖性抗原的T细胞依赖性体液免疫反应。目前，除了依赖抗原呈递细胞表达CD1d和/或NKT亚群的发育外，关于其他涉及的机制知之甚少。因此，我们检验了这样一个假设，即B细胞直接呈递α-GC是NKT增强针对T依赖性抗原的抗体反应所必需的。在用单独的NP-KLH或与α-GC混合的NP-KLH免疫之前，我们用来自C57Bl/6供体或CD1d（-/-）供体的B细胞重建了B细胞缺陷的microMT小鼠。我们有一个惊人的发现，即NKT增强的抗体反应绝对需要B细胞表达CD1d。我们的数据表明，NKT细胞增强体液免疫反应的机制涉及与表达CD1d的B细胞相互作用。
