Malakhova Margarita, Tereshko Valentina, Lee Sung-Young, Yao Ke, Cho Yong-Yeon, Bode Ann, Dong Zigang
The Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, Minnesota 55912, USA.
Nat Struct Mol Biol. 2008 Jan;15(1):112-3. doi: 10.1038/nsmb1347. Epub 2007 Dec 16.
The X-ray structure at 2.0-A resolution of the p90 ribosomal S6 kinase 2 C-terminal kinase domain revealed a C-terminal autoinhibitory alphaL-helix that was embedded in the kinase scaffold and determines the inactive kinase conformation. We suggest a mechanism of activation through displacement of the alphaL-helix and rearrangement of the conserved residue Glu500, as well as the reorganization of the T-loop into the active conformation.
p90核糖体S6激酶2 C端激酶结构域2.0埃分辨率的X射线结构揭示了一个C端自抑制αL螺旋,该螺旋嵌入激酶支架并决定了激酶的无活性构象。我们提出了一种激活机制,即通过αL螺旋的移位和保守残基Glu500的重排,以及T环重排为活性构象来实现激活。