非洲爪蟾卵母细胞伤口愈合作为分析微管-肌动蛋白相互作用的模型系统。

Xenopus oocyte wound healing as a model system for analysis of microtubule-actin interactions.

作者信息

Zhang Tong, Mandato Craig A

机构信息

McGill University, Montreal, Canada.

出版信息

Methods Mol Med. 2007;137:181-8. doi: 10.1007/978-1-59745-442-1_13.

DOI:10.1007/978-1-59745-442-1_13

PMID:18085230

Abstract

Microtubule-actin interactions are fundamental to many cellular processes such as cytokinesis and cellular locomotion. Investigating the mechanism of microtubule-actin interactions is the key to understand the cellular morphogenesis and related pathological processes. The abundance and highly dynamic nature of microtubules and F-actin raise a serious challenge when trying to distinguish between the real and fortuitous interactions within a cell. Xenopus oocyte wound model represents an ideal system to study microtubule-actin interactions as well as microtubule-dependent control of the actin polymerization. Here, we describe a series of cytoskeleton specific treatments in Xenopus oocyte wound healing experiments and use confocal fluorescence microscopy to analyze fixed oocytes to examine microtubule-actin interactions.

摘要

微管与肌动蛋白的相互作用对于许多细胞过程（如胞质分裂和细胞运动）至关重要。研究微管与肌动蛋白相互作用的机制是理解细胞形态发生及相关病理过程的关键。微管和F-肌动蛋白的丰富性和高度动态性使得在区分细胞内真实和偶然的相互作用时面临严峻挑战。非洲爪蟾卵母细胞伤口模型是研究微管与肌动蛋白相互作用以及微管依赖性肌动蛋白聚合控制的理想系统。在此，我们描述了非洲爪蟾卵母细胞伤口愈合实验中的一系列细胞骨架特异性处理，并使用共聚焦荧光显微镜分析固定的卵母细胞以检查微管与肌动蛋白的相互作用。